[W::hts_idx_load3] The index file is older than the data file: 659_bua-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam.bai
Reading reference bed file:  ref_annot.bed  ...  Done
Load BAM file ...  Done

===================================================================
Total splicing  Events:	336039954
Known Splicing Events:	333585537
Partial Novel Splicing Events:	1498872
Novel Splicing Events:	781779
Filtered Splicing Events:	173766

Total splicing  Junctions:	587239
Known Splicing Junctions:	281574
Partial Novel Splicing Junctions:	167522
Novel Splicing Junctions:	138143

===================================================================
Create BED file ...
Create Interact file ...