Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/64/53cf39f6294566a8d9df277ab40e56/659_bua-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam.bai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/4a/0243baf59143a09530ee8911e13e43/ref_annot.bed
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/37/b7d3a553ebbbee2474d5f4a1525852/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/37/b7d3a553ebbbee2474d5f4a1525852/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/d9/d6b21777eaa44be50abd18de7f4251/659_bua-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam
==> STAGING COMPLETE (5 inputs)

[W::hts_idx_load3] The index file is older than the data file: 659_bua-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (16793178) splicing reads. 252966 splicing junctions. 176026 known splicing junctions. 76940 novel splicing junctions.
sampling 10% (33586356) splicing reads. 313368 splicing junctions. 189059 known splicing junctions. 124309 novel splicing junctions.
sampling 15% (50379534) splicing reads. 357134 splicing junctions. 196394 known splicing junctions. 160740 novel splicing junctions.
sampling 20% (67172712) splicing reads. 391828 splicing junctions. 201140 known splicing junctions. 190688 novel splicing junctions.
sampling 25% (83965890) splicing reads. 420757 splicing junctions. 204783 known splicing junctions. 215974 novel splicing junctions.
sampling 30% (100759068) splicing reads. 444997 splicing junctions. 207419 known splicing junctions. 237578 novel splicing junctions.
sampling 35% (117552247) splicing reads. 466502 splicing junctions. 209604 known splicing junctions. 256898 novel splicing junctions.
sampling 40% (134345425) splicing reads. 484762 splicing junctions. 211315 known splicing junctions. 273447 novel splicing junctions.
sampling 45% (151138603) splicing reads. 500921 splicing junctions. 212759 known splicing junctions. 288162 novel splicing junctions.
sampling 50% (167931781) splicing reads. 514954 splicing junctions. 213970 known splicing junctions. 300984 novel splicing junctions.
sampling 55% (184724959) splicing reads. 527549 splicing junctions. 215079 known splicing junctions. 312470 novel splicing junctions.
sampling 60% (201518137) splicing reads. 538551 splicing junctions. 215998 known splicing junctions. 322553 novel splicing junctions.
sampling 65% (218311315) splicing reads. 548099 splicing junctions. 216753 known splicing junctions. 331346 novel splicing junctions.
sampling 70% (235104494) splicing reads. 556518 splicing junctions. 217400 known splicing junctions. 339118 novel splicing junctions.
sampling 75% (251897672) splicing reads. 563834 splicing junctions. 217989 known splicing junctions. 345845 novel splicing junctions.
sampling 80% (268690850) splicing reads. 570191 splicing junctions. 218492 known splicing junctions. 351699 novel splicing junctions.
sampling 85% (285484028) splicing reads. 575765 splicing junctions. 218937 known splicing junctions. 356828 novel splicing junctions.
sampling 90% (302277206) splicing reads. 580438 splicing junctions. 219251 known splicing junctions. 361187 novel splicing junctions.
sampling 95% (319070384) splicing reads. 584176 splicing junctions. 219527 known splicing junctions. 364649 novel splicing junctions.
sampling 100% (335863563) splicing reads. 587081 splicing junctions. 219760 known splicing junctions. 367321 novel splicing junctions.
null device 
          1