+ mkdir 659_bGW-T1-TRNA-1_B23MHV2LT4_1 + all_qc -bam 659_bGW-T1-TRNA-1_B23MHV2LT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder 659_bGW-T1-TRNA-1_B23MHV2LT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: 659_bGW-T1-TRNA-1_B23MHV2LT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: 659_bGW-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] [W::hts_idx_load3] The index file is older than the data file: 659_bGW-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bGW-T1-TRNA-1_B23MHV2LT4_1.md.bai The index file is older than the data file: 659_bGW-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bGW-T1-TRNA-1_B23MHV2LT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: 659_bGW-T1-TRNA-1_B23MHV2LT4_1.md.bai 0it [00:00, ?it/s][W::hts_idx_load3] The index file is older than the data file: 659_bGW-T1-TRNA-1_B23MHV2LT4_1.md.bai 140413it [00:00, 1404034.26it/s] 291504it [00:00, 1466619.62it/s] 446644it [00:00, 1505303.58it/s] 601118it [00:00, 1520850.46it/s] 753204it [00:00, 1233892.18it/s] 883787it [00:00, 1168012.93it/s] 1034688it [00:00, 1263911.11it/s] 1182280it [00:00, 1324705.48it/s] 1326821it [00:00, 1359776.48it/s] 1472915it [00:01, 1389398.58it/s] 1620537it [00:01, 1415028.09it/s] 1768842it [00:01, 1435197.39it/s] 1914198it [00:01, 1440647.82it/s] 2060821it [00:01, 1448267.98it/s] 2208634it [00:01, 1457103.69it/s] 2356908it [00:01, 1464759.57it/s] 2503683it [00:01, 1458443.18it/s] 2649741it [00:01, 1454712.72it/s] 2795362it [00:01, 1453337.20it/s] 2940800it [00:02, 1451549.20it/s] 3087078it [00:02, 1454892.98it/s] 3233627it [00:02, 1457976.61it/s] 3379610it [00:02, 1458517.27it/s] 3525489it [00:02, 1457606.29it/s] 3671269it [00:02, 1455585.70it/s] 3816841it [00:02, 1418227.26it/s] 3958878it [00:02, 1347355.35it/s] 4094402it [00:02, 1311887.55it/s] 4227325it [00:03, 1316778.50it/s] 4359459it [00:03, 1294595.46it/s] 4490568it [00:03, 1299324.31it/s] 4621265it [00:03, 1301532.06it/s] 4764339it [00:03, 1339508.54it/s] 4906794it [00:03, 1364641.57it/s] 5047262it [00:03, 1376512.56it/s] 5190071it [00:03, 1391858.08it/s] 5333647it [00:03, 1404942.82it/s] 5476490it [00:03, 1411946.09it/s] 5617947it [00:04, 1412647.10it/s] 5762133it [00:04, 1421212.53it/s] 5904285it [00:04, 1417633.19it/s] 6046241it [00:04, 1418090.70it/s] 6188453it [00:04, 1419282.32it/s] 6330393it [00:04, 1408184.57it/s] 6471238it [00:04, 1404749.23it/s] 6611732it [00:04, 1403598.38it/s] 6753326it [00:04, 1407168.57it/s] 6895047it [00:04, 1410142.52it/s] 7043335it [00:05, 1431896.07it/s] 7191489it [00:05, 1446753.03it/s] 7339942it [00:05, 1458059.96it/s] 7485757it [00:05, 1444280.62it/s] 7631103it [00:05, 1447000.47it/s] 7775830it [00:05, 1441023.00it/s] 7920813it [00:05, 1443637.04it/s] 8065194it [00:05, 1398017.33it/s] 8211147it [00:05, 1415990.80it/s] 8358230it [00:05, 1432133.70it/s] 8509923it [00:06, 1457238.17it/s] 8662820it [00:06, 1478475.14it/s] 8815801it [00:06, 1493765.22it/s] 8967861it [00:06, 1501614.73it/s] 9118339it [00:06, 1502543.51it/s] 9268651it [00:06, 1502092.60it/s] 9418901it [00:06, 1501088.70it/s] 9569038it [00:06, 1498190.15it/s] 9718878it [00:06, 1494910.23it/s] 9868470it [00:06, 1495059.09it/s] 10017987it [00:07, 1487344.89it/s] 10166737it [00:07, 1474030.69it/s] 10314171it [00:07, 1463728.74it/s] 10460573it [00:07, 1455298.99it/s] 10610500it [00:07, 1468149.81it/s] 10761513it [00:07, 1480611.83it/s] 10909607it [00:07, 1476239.98it/s] 11057255it [00:07, 1464423.47it/s] 11203728it [00:07, 1462107.18it/s] 11351793it [00:07, 1467611.82it/s] 11498573it [00:08, 1248665.33it/s] 11629048it [00:08, 1255187.35it/s] 11765908it [00:08, 1286217.31it/s] 11902373it [00:08, 1308274.95it/s] 12048698it [00:08, 1352697.82it/s] 12197526it [00:08, 1391940.33it/s] 12347614it [00:08, 1423877.87it/s] 12496321it [00:08, 1442526.67it/s] 12647207it [00:08, 1462197.61it/s] 12798815it [00:09, 1478227.63it/s] 12954188it [00:09, 1500741.24it/s] 13110269it [00:09, 1518681.31it/s][W::hts_idx_load3] The index file is older than the data file: 659_bGW-T1-TRNA-1_B23MHV2LT4_1.md.bai 13268475it [00:09, 1537588.23it/s] 13422724it [00:09, 1538991.57it/s] 13576732it [00:09, 1523433.22it/s] 13729186it [00:09, 1519460.02it/s] 13882310it [00:09, 1522955.25it/s] 14037190it [00:09, 1530512.44it/s] 14191418it [00:09, 1534017.44it/s] 14344852it [00:10, 1533147.63it/s] 14502004it [00:10, 1544586.48it/s] 14659639it [00:10, 1554087.91it/s] 14816990it [00:10, 1559897.02it/s] 14974367it [00:10, 1563941.92it/s] 15131680it [00:10, 1566685.86it/s] 15290473it [00:10, 1573040.97it/s] 15447782it [00:10, 1554530.20it/s] 15603287it [00:10, 1551101.42it/s] 15758433it [00:10, 1541962.97it/s] 15912660it [00:11, 1531146.08it/s] 16065804it [00:11, 1504253.96it/s] 16143740it [00:11, 1440280.47it/s] [W::hts_idx_load3] The index file is older than the data file: 659_bGW-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bGW-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bGW-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bGW-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bGW-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bGW-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bGW-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bGW-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bGW-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bGW-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bGW-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bGW-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bGW-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bGW-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bGW-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bGW-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bGW-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bGW-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bGW-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bGW-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: 659_bGW-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bGW-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bGW-T1-TRNA-1_B23MHV2LT4_1.md.bai + merge_qc -folders 659_bGW-T1-TRNA-1_B23MHV2LT4_1/659_bGW-T1-TRNA-1_B23MHV2LT4_1.md -output 659_bGW-T1-TRNA-1_B23MHV2LT4_1/659_bGW-T1-TRNA-1_B23MHV2LT4_1_full_basic_stats.tsv + head -n1 659_bGW-T1-TRNA-1_B23MHV2LT4_1/659_bGW-T1-TRNA-1_B23MHV2LT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed 659_bGW-T1-TRNA-1_B23MHV2LT4_1/659_bGW-T1-TRNA-1_B23MHV2LT4_1_full_basic_stats.tsv