+ mkdir 659_bMa-T1-TRNA-1_B23MHV2LT4_1 + all_qc -bam 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder 659_bMa-T1-TRNA-1_B23MHV2LT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai 0it [00:00, ?it/s] 133978it [00:00, 1339666.29it/s] 284194it [00:00, 1435161.75it/s] 442535it [00:00, 1502788.38it/s] 608959it [00:00, 1566483.37it/s] 777859it [00:00, 1610609.36it/s] 945569it [00:00, 1633175.77it/s] 1108887it [00:00, 1563639.41it/s] 1265812it [00:00, 1549023.84it/s] 1429256it [00:00, 1574954.29it/s] 1595294it [00:01, 1600775.23it/s] 1765257it [00:01, 1630584.93it/s] 1933991it [00:01, 1647593.77it/s] 2098929it [00:01, 1642424.79it/s] 2269070it [00:01, 1660072.32it/s] 2440248it [00:01, 1675537.67it/s] 2610002it [00:01, 1682095.70it/s] 2779254it [00:01, 1685180.89it/s] 2947813it [00:01, 1679148.32it/s][W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai 3117130it [00:01, 1683308.20it/s] 3285485it [00:02, 1665215.61it/s] 3452064it [00:02, 1635724.86it/s] 3615769it [00:02, 1628242.60it/s] 3778680it [00:02, 1606689.42it/s] 3940723it [00:02, 1610669.19it/s] 4101863it [00:02, 1584093.06it/s] 4267938it [00:02, 1603129.99it/s] 4428364it [00:02, 1588239.08it/s] 4598195it [00:02, 1620531.94it/s] 4770669it [00:02, 1651332.82it/s][W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai 4939526it [00:03, 1662222.03it/s] 5111235it [00:03, 1678413.73it/s] 5279156it [00:03, 1677517.44it/s] 5449754it [00:03, 1685911.75it/s] 5618387it [00:03, 1684487.28it/s] 5788750it [00:03, 1690063.00it/s] 5961584it [00:03, 1701422.36it/s] 6132306it [00:03, 1703102.67it/s] 6302629it [00:03, 1701501.13it/s] 6472788it [00:03, 1660439.89it/s] 6639053it [00:04, 1480371.52it/s] 6790705it [00:04, 1371886.86it/s] 6931426it [00:04, 1307081.69it/s] 7064682it [00:04, 1260209.32it/s] 7192376it [00:04, 1242557.48it/s] 7317662it [00:04, 1227281.69it/s] 7441012it [00:04, 1222399.25it/s] 7563653it [00:04, 1215439.88it/s] 7685445it [00:04, 1205552.80it/s] 7806322it [00:05, 1206447.55it/s] 7927073it [00:05, 1193407.72it/s] 8046486it [00:05, 1183098.55it/s] 8113870it [00:05, 1514749.63it/s] [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bMa-T1-TRNA-1_B23MHV2LT4_1.md.bai + merge_qc -folders 659_bMa-T1-TRNA-1_B23MHV2LT4_1/659_bMa-T1-TRNA-1_B23MHV2LT4_1.md -output 659_bMa-T1-TRNA-1_B23MHV2LT4_1/659_bMa-T1-TRNA-1_B23MHV2LT4_1_full_basic_stats.tsv + head -n1 659_bMa-T1-TRNA-1_B23MHV2LT4_1/659_bMa-T1-TRNA-1_B23MHV2LT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed 659_bMa-T1-TRNA-1_B23MHV2LT4_1/659_bMa-T1-TRNA-1_B23MHV2LT4_1_full_basic_stats.tsv