[W::hts_idx_load3] The index file is older than the data file: 659_b4x-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (2525943) splicing reads. 147796 splicing junctions. 133729 known splicing junctions. 14067 novel splicing junctions.
sampling 10% (5051887) splicing reads. 161869 splicing junctions. 142439 known splicing junctions. 19430 novel splicing junctions.
sampling 15% (7577830) splicing reads. 168190 splicing junctions. 145830 known splicing junctions. 22360 novel splicing junctions.
sampling 20% (10103774) splicing reads. 171864 splicing junctions. 147671 known splicing junctions. 24193 novel splicing junctions.
sampling 25% (12629718) splicing reads. 174323 splicing junctions. 148827 known splicing junctions. 25496 novel splicing junctions.
sampling 30% (15155661) splicing reads. 176166 splicing junctions. 149657 known splicing junctions. 26509 novel splicing junctions.
sampling 35% (17681605) splicing reads. 177544 splicing junctions. 150238 known splicing junctions. 27306 novel splicing junctions.
sampling 40% (20207549) splicing reads. 178688 splicing junctions. 150720 known splicing junctions. 27968 novel splicing junctions.
sampling 45% (22733492) splicing reads. 179616 splicing junctions. 151105 known splicing junctions. 28511 novel splicing junctions.
sampling 50% (25259436) splicing reads. 180311 splicing junctions. 151349 known splicing junctions. 28962 novel splicing junctions.
sampling 55% (27785380) splicing reads. 180925 splicing junctions. 151566 known splicing junctions. 29359 novel splicing junctions.
sampling 60% (30311323) splicing reads. 181430 splicing junctions. 151747 known splicing junctions. 29683 novel splicing junctions.
sampling 65% (32837267) splicing reads. 181847 splicing junctions. 151888 known splicing junctions. 29959 novel splicing junctions.
sampling 70% (35363211) splicing reads. 182209 splicing junctions. 151995 known splicing junctions. 30214 novel splicing junctions.
sampling 75% (37889154) splicing reads. 182511 splicing junctions. 152103 known splicing junctions. 30408 novel splicing junctions.
sampling 80% (40415098) splicing reads. 182744 splicing junctions. 152177 known splicing junctions. 30567 novel splicing junctions.
sampling 85% (42941042) splicing reads. 182945 splicing junctions. 152249 known splicing junctions. 30696 novel splicing junctions.
sampling 90% (45466985) splicing reads. 183088 splicing junctions. 152296 known splicing junctions. 30792 novel splicing junctions.
sampling 95% (47992929) splicing reads. 183184 splicing junctions. 152317 known splicing junctions. 30867 novel splicing junctions.
sampling 100% (50518873) splicing reads. 183237 splicing junctions. 152332 known splicing junctions. 30905 novel splicing junctions.