Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/4a/0243baf59143a09530ee8911e13e43/ref_annot.bed
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/07/8bc2c41a9924548708a2687a51752d/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/be/27385695e43288cff28ebcd46dc4c9/659_bTo-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/07/8bc2c41a9924548708a2687a51752d/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ec/dfbb32fd89ba107e4305d79a2576c9/659_bTo-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam.bai
==> STAGING COMPLETE (5 inputs)

[W::hts_idx_load3] The index file is older than the data file: 659_bTo-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (18709618) splicing reads. 261217 splicing junctions. 182444 known splicing junctions. 78773 novel splicing junctions.
sampling 10% (37419237) splicing reads. 324269 splicing junctions. 195960 known splicing junctions. 128309 novel splicing junctions.
sampling 15% (56128855) splicing reads. 370237 splicing junctions. 203385 known splicing junctions. 166852 novel splicing junctions.
sampling 20% (74838474) splicing reads. 406841 splicing junctions. 208349 known splicing junctions. 198492 novel splicing junctions.
sampling 25% (93548093) splicing reads. 437600 splicing junctions. 211971 known splicing junctions. 225629 novel splicing junctions.
sampling 30% (112257711) splicing reads. 463782 splicing junctions. 214672 known splicing junctions. 249110 novel splicing junctions.
sampling 35% (130967330) splicing reads. 486436 splicing junctions. 216912 known splicing junctions. 269524 novel splicing junctions.
sampling 40% (149676949) splicing reads. 506240 splicing junctions. 218757 known splicing junctions. 287483 novel splicing junctions.
sampling 45% (168386567) splicing reads. 523689 splicing junctions. 220294 known splicing junctions. 303395 novel splicing junctions.
sampling 50% (187096186) splicing reads. 539255 splicing junctions. 221566 known splicing junctions. 317689 novel splicing junctions.
sampling 55% (205805805) splicing reads. 552874 splicing junctions. 222671 known splicing junctions. 330203 novel splicing junctions.
sampling 60% (224515423) splicing reads. 565099 splicing junctions. 223594 known splicing junctions. 341505 novel splicing junctions.
sampling 65% (243225042) splicing reads. 575878 splicing junctions. 224406 known splicing junctions. 351472 novel splicing junctions.
sampling 70% (261934661) splicing reads. 585573 splicing junctions. 225114 known splicing junctions. 360459 novel splicing junctions.
sampling 75% (280644279) splicing reads. 594004 splicing junctions. 225697 known splicing junctions. 368307 novel splicing junctions.
sampling 80% (299353898) splicing reads. 601584 splicing junctions. 226227 known splicing junctions. 375357 novel splicing junctions.
sampling 85% (318063517) splicing reads. 608087 splicing junctions. 226652 known splicing junctions. 381435 novel splicing junctions.
sampling 90% (336773135) splicing reads. 613653 splicing junctions. 227032 known splicing junctions. 386621 novel splicing junctions.
sampling 95% (355482754) splicing reads. 618260 splicing junctions. 227360 known splicing junctions. 390900 novel splicing junctions.
sampling 100% (374192373) splicing reads. 622054 splicing junctions. 227639 known splicing junctions. 394415 novel splicing junctions.
null device 
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