[W::hts_idx_load3] The index file is older than the data file: 659_bTq-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (16525886) splicing reads. 273721 splicing junctions. 190914 known splicing junctions. 82807 novel splicing junctions.
sampling 10% (33051772) splicing reads. 337946 splicing junctions. 204275 known splicing junctions. 133671 novel splicing junctions.
sampling 15% (49577659) splicing reads. 384346 splicing junctions. 211510 known splicing junctions. 172836 novel splicing junctions.
sampling 20% (66103545) splicing reads. 421927 splicing junctions. 216526 known splicing junctions. 205401 novel splicing junctions.
sampling 25% (82629432) splicing reads. 453705 splicing junctions. 220030 known splicing junctions. 233675 novel splicing junctions.
sampling 30% (99155318) splicing reads. 480785 splicing junctions. 222792 known splicing junctions. 257993 novel splicing junctions.
sampling 35% (115681204) splicing reads. 504956 splicing junctions. 225070 known splicing junctions. 279886 novel splicing junctions.
sampling 40% (132207091) splicing reads. 526220 splicing junctions. 226843 known splicing junctions. 299377 novel splicing junctions.
sampling 45% (148732977) splicing reads. 545019 splicing junctions. 228419 known splicing junctions. 316600 novel splicing junctions.
sampling 50% (165258864) splicing reads. 561935 splicing junctions. 229702 known splicing junctions. 332233 novel splicing junctions.
sampling 55% (181784750) splicing reads. 577120 splicing junctions. 230848 known splicing junctions. 346272 novel splicing junctions.
sampling 60% (198310636) splicing reads. 590629 splicing junctions. 231802 known splicing junctions. 358827 novel splicing junctions.
sampling 65% (214836523) splicing reads. 602934 splicing junctions. 232636 known splicing junctions. 370298 novel splicing junctions.
sampling 70% (231362409) splicing reads. 614015 splicing junctions. 233371 known splicing junctions. 380644 novel splicing junctions.
sampling 75% (247888296) splicing reads. 623856 splicing junctions. 234027 known splicing junctions. 389829 novel splicing junctions.
sampling 80% (264414182) splicing reads. 632792 splicing junctions. 234613 known splicing junctions. 398179 novel splicing junctions.
sampling 85% (280940068) splicing reads. 640407 splicing junctions. 235121 known splicing junctions. 405286 novel splicing junctions.
sampling 90% (297465955) splicing reads. 647447 splicing junctions. 235574 known splicing junctions. 411873 novel splicing junctions.
sampling 95% (313991841) splicing reads. 653526 splicing junctions. 235923 known splicing junctions. 417603 novel splicing junctions.
sampling 100% (330517728) splicing reads. 658559 splicing junctions. 236233 known splicing junctions. 422326 novel splicing junctions.