[W::hts_idx_load3] The index file is older than the data file: 659_bIB-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (18803150) splicing reads. 269815 splicing junctions. 186258 known splicing junctions. 83557 novel splicing junctions.
sampling 10% (37606301) splicing reads. 337006 splicing junctions. 199410 known splicing junctions. 137596 novel splicing junctions.
sampling 15% (56409452) splicing reads. 387298 splicing junctions. 206905 known splicing junctions. 180393 novel splicing junctions.
sampling 20% (75212603) splicing reads. 428112 splicing junctions. 211826 known splicing junctions. 216286 novel splicing junctions.
sampling 25% (94015753) splicing reads. 462681 splicing junctions. 215406 known splicing junctions. 247275 novel splicing junctions.
sampling 30% (112818904) splicing reads. 492464 splicing junctions. 218272 known splicing junctions. 274192 novel splicing junctions.
sampling 35% (131622055) splicing reads. 519058 splicing junctions. 220538 known splicing junctions. 298520 novel splicing junctions.
sampling 40% (150425206) splicing reads. 542845 splicing junctions. 222504 known splicing junctions. 320341 novel splicing junctions.
sampling 45% (169228356) splicing reads. 563840 splicing junctions. 224032 known splicing junctions. 339808 novel splicing junctions.
sampling 50% (188031507) splicing reads. 582839 splicing junctions. 225401 known splicing junctions. 357438 novel splicing junctions.
sampling 55% (206834658) splicing reads. 599591 splicing junctions. 226490 known splicing junctions. 373101 novel splicing junctions.
sampling 60% (225637809) splicing reads. 614668 splicing junctions. 227468 known splicing junctions. 387200 novel splicing junctions.
sampling 65% (244440959) splicing reads. 628352 splicing junctions. 228361 known splicing junctions. 399991 novel splicing junctions.
sampling 70% (263244110) splicing reads. 640626 splicing junctions. 229140 known splicing junctions. 411486 novel splicing junctions.
sampling 75% (282047261) splicing reads. 651456 splicing junctions. 229755 known splicing junctions. 421701 novel splicing junctions.
sampling 80% (300850412) splicing reads. 661252 splicing junctions. 230300 known splicing junctions. 430952 novel splicing junctions.
sampling 85% (319653562) splicing reads. 669997 splicing junctions. 230842 known splicing junctions. 439155 novel splicing junctions.
sampling 90% (338456713) splicing reads. 677472 splicing junctions. 231274 known splicing junctions. 446198 novel splicing junctions.
sampling 95% (357259864) splicing reads. 683713 splicing junctions. 231598 known splicing junctions. 452115 novel splicing junctions.
sampling 100% (376063015) splicing reads. 689215 splicing junctions. 231904 known splicing junctions. 457311 novel splicing junctions.