[W::hts_idx_load3] The index file is older than the data file: 659_bQZ-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (17211035) splicing reads. 282311 splicing junctions. 191039 known splicing junctions. 91272 novel splicing junctions.
sampling 10% (34422070) splicing reads. 349604 splicing junctions. 204110 known splicing junctions. 145494 novel splicing junctions.
sampling 15% (51633105) splicing reads. 397326 splicing junctions. 211131 known splicing junctions. 186195 novel splicing junctions.
sampling 20% (68844141) splicing reads. 434419 splicing junctions. 215610 known splicing junctions. 218809 novel splicing junctions.
sampling 25% (86055176) splicing reads. 464522 splicing junctions. 218785 known splicing junctions. 245737 novel splicing junctions.
sampling 30% (103266211) splicing reads. 489878 splicing junctions. 221338 known splicing junctions. 268540 novel splicing junctions.
sampling 35% (120477247) splicing reads. 511232 splicing junctions. 223278 known splicing junctions. 287954 novel splicing junctions.
sampling 40% (137688282) splicing reads. 529785 splicing junctions. 224826 known splicing junctions. 304959 novel splicing junctions.
sampling 45% (154899317) splicing reads. 545846 splicing junctions. 226174 known splicing junctions. 319672 novel splicing junctions.
sampling 50% (172110353) splicing reads. 559713 splicing junctions. 227273 known splicing junctions. 332440 novel splicing junctions.
sampling 55% (189321388) splicing reads. 572149 splicing junctions. 228253 known splicing junctions. 343896 novel splicing junctions.
sampling 60% (206532423) splicing reads. 582949 splicing junctions. 229046 known splicing junctions. 353903 novel splicing junctions.
sampling 65% (223743458) splicing reads. 592408 splicing junctions. 229700 known splicing junctions. 362708 novel splicing junctions.
sampling 70% (240954494) splicing reads. 600804 splicing junctions. 230344 known splicing junctions. 370460 novel splicing junctions.
sampling 75% (258165529) splicing reads. 607921 splicing junctions. 230811 known splicing junctions. 377110 novel splicing junctions.
sampling 80% (275376564) splicing reads. 614008 splicing junctions. 231243 known splicing junctions. 382765 novel splicing junctions.
sampling 85% (292587600) splicing reads. 619318 splicing junctions. 231634 known splicing junctions. 387684 novel splicing junctions.
sampling 90% (309798635) splicing reads. 623684 splicing junctions. 231920 known splicing junctions. 391764 novel splicing junctions.
sampling 95% (327009670) splicing reads. 627269 splicing junctions. 232169 known splicing junctions. 395100 novel splicing junctions.
sampling 100% (344220706) splicing reads. 630148 splicing junctions. 232395 known splicing junctions. 397753 novel splicing junctions.