[W::hts_idx_load3] The index file is older than the data file: 659_bR4-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (14611633) splicing reads. 290242 splicing junctions. 197501 known splicing junctions. 92741 novel splicing junctions.
sampling 10% (29223266) splicing reads. 359358 splicing junctions. 210606 known splicing junctions. 148752 novel splicing junctions.
sampling 15% (43834899) splicing reads. 410015 splicing junctions. 217583 known splicing junctions. 192432 novel splicing junctions.
sampling 20% (58446533) splicing reads. 450718 splicing junctions. 222282 known splicing junctions. 228436 novel splicing junctions.
sampling 25% (73058166) splicing reads. 484512 splicing junctions. 225671 known splicing junctions. 258841 novel splicing junctions.
sampling 30% (87669799) splicing reads. 513883 splicing junctions. 228340 known splicing junctions. 285543 novel splicing junctions.
sampling 35% (102281432) splicing reads. 539412 splicing junctions. 230434 known splicing junctions. 308978 novel splicing junctions.
sampling 40% (116893066) splicing reads. 561758 splicing junctions. 232133 known splicing junctions. 329625 novel splicing junctions.
sampling 45% (131504699) splicing reads. 581399 splicing junctions. 233620 known splicing junctions. 347779 novel splicing junctions.
sampling 50% (146116332) splicing reads. 599077 splicing junctions. 234854 known splicing junctions. 364223 novel splicing junctions.
sampling 55% (160727965) splicing reads. 614697 splicing junctions. 235907 known splicing junctions. 378790 novel splicing junctions.
sampling 60% (175339599) splicing reads. 628601 splicing junctions. 236759 known splicing junctions. 391842 novel splicing junctions.
sampling 65% (189951232) splicing reads. 641035 splicing junctions. 237535 known splicing junctions. 403500 novel splicing junctions.
sampling 70% (204562865) splicing reads. 651914 splicing junctions. 238224 known splicing junctions. 413690 novel splicing junctions.
sampling 75% (219174498) splicing reads. 661587 splicing junctions. 238732 known splicing junctions. 422855 novel splicing junctions.
sampling 80% (233786132) splicing reads. 669848 splicing junctions. 239259 known splicing junctions. 430589 novel splicing junctions.
sampling 85% (248397765) splicing reads. 677124 splicing junctions. 239704 known splicing junctions. 437420 novel splicing junctions.
sampling 90% (263009398) splicing reads. 683226 splicing junctions. 240082 known splicing junctions. 443144 novel splicing junctions.
sampling 95% (277621031) splicing reads. 688304 splicing junctions. 240381 known splicing junctions. 447923 novel splicing junctions.
sampling 100% (292232665) splicing reads. 692380 splicing junctions. 240618 known splicing junctions. 451762 novel splicing junctions.