Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/4a/0243baf59143a09530ee8911e13e43/ref_annot.bed
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/6f/c30f9fe2774a14c0a14e49b8934975/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/6f/c30f9fe2774a14c0a14e49b8934975/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/3c/ec0f82af30d147b2e71579937949ce/659_6i-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/4b/1334352268c0695f37d4ead59565d2/659_6i-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam.bai
==> STAGING COMPLETE (5 inputs)

[W::hts_idx_load3] The index file is older than the data file: 659_6i-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (9449283) splicing reads. 227267 splicing junctions. 178577 known splicing junctions. 48690 novel splicing junctions.
sampling 10% (18898566) splicing reads. 267414 splicing junctions. 190582 known splicing junctions. 76832 novel splicing junctions.
sampling 15% (28347849) splicing reads. 293421 splicing junctions. 196742 known splicing junctions. 96679 novel splicing junctions.
sampling 20% (37797132) splicing reads. 312653 splicing junctions. 200678 known splicing junctions. 111975 novel splicing junctions.
sampling 25% (47246415) splicing reads. 327524 splicing junctions. 203335 known splicing junctions. 124189 novel splicing junctions.
sampling 30% (56695698) splicing reads. 339587 splicing junctions. 205339 known splicing junctions. 134248 novel splicing junctions.
sampling 35% (66144980) splicing reads. 349293 splicing junctions. 206874 known splicing junctions. 142419 novel splicing junctions.
sampling 40% (75594264) splicing reads. 357415 splicing junctions. 208143 known splicing junctions. 149272 novel splicing junctions.
sampling 45% (85043547) splicing reads. 364305 splicing junctions. 209186 known splicing junctions. 155119 novel splicing junctions.
sampling 50% (94492830) splicing reads. 369966 splicing junctions. 209981 known splicing junctions. 159985 novel splicing junctions.
sampling 55% (103942113) splicing reads. 374834 splicing junctions. 210643 known splicing junctions. 164191 novel splicing junctions.
sampling 60% (113391396) splicing reads. 378948 splicing junctions. 211218 known splicing junctions. 167730 novel splicing junctions.
sampling 65% (122840679) splicing reads. 382414 splicing junctions. 211674 known splicing junctions. 170740 novel splicing junctions.
sampling 70% (132289961) splicing reads. 385342 splicing junctions. 212059 known splicing junctions. 173283 novel splicing junctions.
sampling 75% (141739245) splicing reads. 387808 splicing junctions. 212413 known splicing junctions. 175395 novel splicing junctions.
sampling 80% (151188528) splicing reads. 389790 splicing junctions. 212675 known splicing junctions. 177115 novel splicing junctions.
sampling 85% (160637811) splicing reads. 391448 splicing junctions. 212914 known splicing junctions. 178534 novel splicing junctions.
sampling 90% (170087094) splicing reads. 392646 splicing junctions. 213069 known splicing junctions. 179577 novel splicing junctions.
sampling 95% (179536377) splicing reads. 393571 splicing junctions. 213183 known splicing junctions. 180388 novel splicing junctions.
sampling 100% (188985660) splicing reads. 394061 splicing junctions. 213242 known splicing junctions. 180819 novel splicing junctions.
null device 
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