=== DEBUGGING INFORMATION === Current working directory: /tmp/nxf.GcUQ8WNYho Input files: - starfusion_tsv: 659_dkd-T1-TRNA-1_B23MHV2LT4_1.starfusion.starfusion.fusion_predictions.tsv - blacklist: blacklist_hg38_GRCh38_v2.4.0.tsv.gz - filter_config: starfusion_filter_config.yaml - aih_blacklist_breakpoints: blacklist_breakpoint_database_2026-04-27.tsv === PYTHON INFORMATION === /usr/local/bin/python Python 3.11.14 /usr/local/bin/python3 Python 3.11.14 === SCRIPT LOCATION === Checking for filter_starfusion_main.py: /tmp/nxf.GcUQ8WNYho/nextflow-bin/filter_starfusion_main.py filter_starfusion_main.py not in current directory bin/filter_starfusion_main.py not found bin/ directory does not exist === ENVIRONMENT PATH === /tmp/nxf.GcUQ8WNYho/nextflow-bin:/usr/local/bin:/usr/local/sbin:/usr/local/bin:/usr/sbin:/usr/bin:/sbin:/bin === CURRENT DIRECTORY CONTENTS === total 64004 drwx------. 3 1000 root 4096 Jun 8 07:20 . drwxrwxrwx. 4 root root 86 Jun 8 07:20 .. -rw-r--r--. 1 1000 root 0 Jun 8 07:20 .command.err -rw-r--r--. 1 1000 root 881 Jun 8 07:20 .command.out -rw-r--r--. 1 1000 root 14577 Jun 8 07:20 .command.run -rw-r--r--. 1 1000 root 3218 Jun 8 07:20 .command.sh -rw-r--r--. 1 1000 root 0 Jun 8 07:20 .command.trace -rw-r--r--. 1 1000 root 246 Jun 8 07:20 659_dkd-T1-TRNA-1_B23MHV2LT4_1.starfusion.starfusion.fusion_predictions.tsv -rw-r--r--. 1 1000 root 10714998 Jun 8 07:20 blacklist_breakpoint_database_2026-04-27.tsv -rw-r--r--. 1 1000 root 54779787 Jun 8 07:20 blacklist_hg38_GRCh38_v2.4.0.tsv.gz drwxr-xr-x. 3 1000 root 4096 Jun 8 07:20 nextflow-bin -rw-r--r--. 1 1000 root 4128 Jun 8 07:20 starfusion_filter_config.yaml === PROJECT DIRECTORY INFO === NXF_PROJECT_DIR: not set baseDir: not set === Running filter script === ================================================================================ STEP 1: LOADING STAR-FUSION PREDICTIONS ================================================================================ Loading STAR-Fusion predictions from: 659_dkd-T1-TRNA-1_B23MHV2LT4_1.starfusion.starfusion.fusion_predictions.tsv ✓ Loaded: 0 fusions ================================================================================ STEP 2: CONVERTING TO BREAKPOINT_ID FORMAT ================================================================================ ✓ Converted to breakpoint_ID format: 0 valid, 0 invalid ================================================================================ STEP 3: FLAGGING WITH BLACKLIST ================================================================================ Loading blacklist (this may take a while)... Loading blacklist from: blacklist_hg38_GRCh38_v2.4.0.tsv.gz Loaded 9381 single rules and 16721785 pair rules Indexed blacklist (bin_size=100000) Flagging fusions with blacklist... ✓ Flagged 0.0 of 0 fusions as blacklisted ================================================================================ STEP 3b: AIH CURATED BREAKPOINT BLACKLIST ================================================================================ Loading AIH blacklist table from: blacklist_breakpoint_database_2026-04-27.tsv Qualified breakpoint keys (orientations): 1378 AIH curated blacklist: matched 0 fusion rows (breakpoint_ID in qualified set) ================================================================================ STEP 4: APPLYING YAML-BASED FILTER ================================================================================ ✓ Filter initialized from: starfusion_filter_config.yaml Applying filter criteria (adding CALL column)... ✓ Filter applied: 0 pass (CALL=1), 0 fail (CALL=0) Total fusions: 0 (all rows retained) Added FILTER column: PASS=0, FAIL=0 ================================================================================ STEP 5: GENERATING SUMMARY STATISTICS ================================================================================ ================================================================================ FILTERING SUMMARY ================================================================================ Total fusions: 0 Pass (CALL=1): 0 (nan%) Fail (CALL=0): 0 (nan%) Read Count Statistics: Fusions with < 3 total reads: 0 Of these, 0 have CALL=0 ================================================================================ STEP 6: EXPORTING RESULTS ================================================================================ ✓ Results exported to: 659_dkd-T1-TRNA-1_B23MHV2LT4_1.starfusion.starfusion.fusion_predictions.filtered.tsv Total fusions: 0 CALL=1 (pass): 0 CALL=0 (fail): 0 ✓ Empty pass_only file created (no passing fusions): 659_dkd-T1-TRNA-1_B23MHV2LT4_1.starfusion.starfusion.fusion_predictions.filtered_pass_only.tsv ✓ Summary exported to: 659_dkd-T1-TRNA-1_B23MHV2LT4_1.starfusion.starfusion.fusion_predictions.filter_summary.txt ================================================================================ ✓ FILTERING COMPLETE ================================================================================