[W::hts_idx_load3] The index file is older than the data file: 659_bTo-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam.bai
Reading reference bed file:  ref_annot.bed  ...  Done
Load BAM file ...  Done

===================================================================
Total splicing  Events:	374284265
Known Splicing Events:	371683317
Partial Novel Splicing Events:	1436633
Novel Splicing Events:	1074421
Filtered Splicing Events:	89894

Total splicing  Junctions:	622171
Known Splicing Junctions:	291087
Partial Novel Splicing Junctions:	173557
Novel Splicing Junctions:	157527

===================================================================
Create BED file ...
Create Interact file ...