STAR --genomeDir ref_genome.fa.star.idx --readFilesIn input1/NTC_0001_0001_B23MHV2LT4_1_1.fastp.fastq.gz input2/NTC_0001_0001_B23MHV2LT4_1_2.fastp.fastq.gz --runThreadN 24 --outFileNamePrefix NTC_0001_0001_B23MHV2LT4_1. --sjdbGTFfile ref_annot.gtf --outSAMattrRGline ID:NTC_0001_0001_B23MHV2LT4_1 SM:NTC_0001_0001_B23MHV2LT4_1 --twopassMode None --chimSegmentMin 12 --chimJunctionOverhangMin 5 --chimMultimapNmax 50 --chimScoreJunctionNonGTAG -4 --chimScoreSeparation 1 --alignIntronMax 140000 --outReadsUnmapped None --readFilesCommand zcat --outSAMtype BAM SortedByCoordinate --outSAMstrandField intronMotif --outSAMunmapped Within --chimOutJunctionFormat 1 --alignSJDBoverhangMin 10 --alignMatesGapMax 100000 --alignSJstitchMismatchNmax 5 -1 5 5 --chimMultimapScoreRange 3 --chimNonchimScoreDropMin 10 --peOverlapNbasesMin 12 --peOverlapMMp 0.1 --alignInsertionFlush Right --alignSplicedMateMapLmin 30 --alignSplicedMateMapLminOverLmate 0.5 --quantMode GeneCounts --runRNGseed 1 --chimOutType Junctions WithinBAM --limitBAMsortRAM 38654705664 STAR version: 2.7.10a compiled: 2022-01-14T18:50:00-05:00 :/home/dobin/data/STAR/STARcode/STAR.master/source Jun 08 07:14:18 ..... started STAR run Jun 08 07:14:18 ..... loading genome Jun 08 07:14:39 ..... processing annotations GTF Jun 08 07:14:51 ..... inserting junctions into the genome indices Jun 08 07:16:15 ..... started mapping Jun 08 07:16:27 ..... finished mapping Jun 08 07:16:29 ..... started sorting BAM Jun 08 07:16:29 ..... finished successfully