Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/21/d9d8d028a93fb2aacbc1210f64b807/.command.sh
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/e5/abeb48a611b93d34ee7291e2d34ab0/659_dm4-T1-TRNA-1_B23MHV2LT4_1.Chimeric.out.junction
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/6e/178ffd1b88e2e66af803d7e40ed9eb/659_dm4-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam.bai
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/21/d9d8d028a93fb2aacbc1210f64b807/.command.run
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/e5/abeb48a611b93d34ee7291e2d34ab0/659_dm4-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/e5/abeb48a611b93d34ee7291e2d34ab0/659_dm4-T1-TRNA-1_B23MHV2LT4_1.SJ.out.tab
==> STAGING COMPLETE (7 inputs)
08:06:08 : INFO : -reading targets list: ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu
-mapping reads to genes
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[6710000], rate=16104000.00/min 08:06:41 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file 659_dm4-T1-TRNA-1_B23MHV2LT4_1.introns --ctat_genome_lib ctat_genome_lib_build_dir --intron_col 0 > 659_dm4-T1-TRNA-1_B23MHV2LT4_1.cancer.introns.prelim
Use of uninitialized value $ctat_genome_lib in concatenation (.) or string at /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl line 18.
-659_dm4-T1-TRNA-1_B23MHV2LT4_1.introns: identified 2209 cancer introns
08:06:41 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file 659_dm4-T1-TRNA-1_B23MHV2LT4_1.introns --ctat_genome_lib ctat_genome_lib_build_dir --intron_col 0 > 659_dm4-T1-TRNA-1_B23MHV2LT4_1.cancer.introns.prelim
08:06:41 : INFO : Running: touch /tmp/nxf.j8dGJ2viId/659_dm4-T1-TRNA-1_B23MHV2LT4_1.chckpts/prelim_introns.ok
08:06:41 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates 659_dm4-T1-TRNA-1_B23MHV2LT4_1.cancer.introns.prelim --min_total_reads 5 > 659_dm4-T1-TRNA-1_B23MHV2LT4_1.cancer.introns
08:06:42 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates 659_dm4-T1-TRNA-1_B23MHV2LT4_1.cancer.introns.prelim --min_total_reads 5 > 659_dm4-T1-TRNA-1_B23MHV2LT4_1.cancer.introns
08:06:42 : INFO : Running: touch /tmp/nxf.j8dGJ2viId/659_dm4-T1-TRNA-1_B23MHV2LT4_1.chckpts/introns_filtered.ok
08:06:42 : INFO : -found 1201 cancer introns
08:06:42 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns 659_dm4-T1-TRNA-1_B23MHV2LT4_1.introns --cancer_introns 659_dm4-T1-TRNA-1_B23MHV2LT4_1.cancer.introns --genome_lib_dir ctat_genome_lib_build_dir --output_bed 659_dm4-T1-TRNA-1_B23MHV2LT4_1.introns.for_IGV.bed
08:06:42 : INFO : Creating the BED File.
08:06:46 : INFO : Saving Bed File as 659_dm4-T1-TRNA-1_B23MHV2LT4_1.introns.for_IGV.bed
08:06:46 : INFO : Execution Time = 0.06 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns 659_dm4-T1-TRNA-1_B23MHV2LT4_1.introns --cancer_introns 659_dm4-T1-TRNA-1_B23MHV2LT4_1.cancer.introns --genome_lib_dir ctat_genome_lib_build_dir --output_bed 659_dm4-T1-TRNA-1_B23MHV2LT4_1.introns.for_IGV.bed
08:06:46 : INFO : Running: touch /tmp/nxf.j8dGJ2viId/659_dm4-T1-TRNA-1_B23MHV2LT4_1.chckpts/intron_igv_bed.ok
08:06:46 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed 659_dm4-T1-TRNA-1_B23MHV2LT4_1.introns.for_IGV.bed --bam 659_dm4-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam --output_prefix 659_dm4-T1-TRNA-1_B23MHV2LT4_1
[W::hts_idx_load3] The index file is older than the data file: 659_dm4-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam.bai
08:15:25 : INFO : Execution Time = 8.66 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed 659_dm4-T1-TRNA-1_B23MHV2LT4_1.introns.for_IGV.bed --bam 659_dm4-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam --output_prefix 659_dm4-T1-TRNA-1_B23MHV2LT4_1
08:15:25 : INFO : Running: touch /tmp/nxf.j8dGJ2viId/659_dm4-T1-TRNA-1_B23MHV2LT4_1.chckpts/reads_alignments_extracted.ok
08:15:26 : INFO : Running: samtools sort -o 659_dm4-T1-TRNA-1_B23MHV2LT4_1.cancer_intron_reads.sorted.bam 659_dm4-T1-TRNA-1_B23MHV2LT4_1.cancer_intron_reads.bam
08:15:26 : INFO : Execution Time = 0.00 minutes. CMD: samtools sort -o 659_dm4-T1-TRNA-1_B23MHV2LT4_1.cancer_intron_reads.sorted.bam 659_dm4-T1-TRNA-1_B23MHV2LT4_1.cancer_intron_reads.bam
08:15:26 : INFO : Running: touch /tmp/nxf.j8dGJ2viId/659_dm4-T1-TRNA-1_B23MHV2LT4_1.chckpts/sort_cancer_intron_reads.ok
08:15:26 : INFO : Running: samtools sort -o 659_dm4-T1-TRNA-1_B23MHV2LT4_1.gene_reads.sorted.bam 659_dm4-T1-TRNA-1_B23MHV2LT4_1.gene_reads.bam
[bam_sort_core] merging from 42 files and 1 in-memory blocks...
08:24:48 : INFO : Execution Time = 9.37 minutes. CMD: samtools sort -o 659_dm4-T1-TRNA-1_B23MHV2LT4_1.gene_reads.sorted.bam 659_dm4-T1-TRNA-1_B23MHV2LT4_1.gene_reads.bam
08:24:48 : INFO : Running: touch /tmp/nxf.j8dGJ2viId/659_dm4-T1-TRNA-1_B23MHV2LT4_1.chckpts/sort_gene_reads.ok
08:24:48 : INFO : Running: /usr/local/src/CTAT-SPLICING/bamsifter/bamsifter -c 50 -i 50 -o 659_dm4-T1-TRNA-1_B23MHV2LT4_1.gene_reads.sorted.sifted.bam.tmp --keep_secondary 659_dm4-T1-TRNA-1_B23MHV2LT4_1.gene_reads.sorted.bam
Done with chr chr1.
Done with chr chr2.
Done with chr chr3.
Done with chr chr4.
Done with chr chr5.
Done with chr chr6.
Done with chr chr7.
Done with chr chr8.
Done with chr chr9.
Done with chr chr10.
Done with chr chr11.
Done with chr chr12.
Done with chr chr13.
Done with chr chr14.
Done with chr chr15.
Done with chr chr16.
Done with chr chr17.
Done with chr chr18.
Done with chr chr19.
Done with chr chr20.
Done with chr chr21.
Done with chr chr22.
Done with chr chrX.
08:27:11 : INFO : Execution Time = 2.39 minutes. CMD: /usr/local/src/CTAT-SPLICING/bamsifter/bamsifter -c 50 -i 50 -o 659_dm4-T1-TRNA-1_B23MHV2LT4_1.gene_reads.sorted.sifted.bam.tmp --keep_secondary 659_dm4-T1-TRNA-1_B23MHV2LT4_1.gene_reads.sorted.bam
08:27:11 : INFO : Running: touch /tmp/nxf.j8dGJ2viId/659_dm4-T1-TRNA-1_B23MHV2LT4_1.chckpts/659_dm4-T1-TRNA-1_B23MHV2LT4_1.gene_reads.sorted.sifted.bam.tmp.ok
08:27:11 : INFO : Running: samtools sort -o 659_dm4-T1-TRNA-1_B23MHV2LT4_1.gene_reads.sorted.sifted.bam 659_dm4-T1-TRNA-1_B23MHV2LT4_1.gene_reads.sorted.sifted.bam.tmp
[bam_sort_core] merging from 2 files and 1 in-memory blocks...
08:27:44 : INFO : Execution Time = 0.55 minutes. CMD: samtools sort -o 659_dm4-T1-TRNA-1_B23MHV2LT4_1.gene_reads.sorted.sifted.bam 659_dm4-T1-TRNA-1_B23MHV2LT4_1.gene_reads.sorted.sifted.bam.tmp
08:27:44 : INFO : Running: touch /tmp/nxf.j8dGJ2viId/659_dm4-T1-TRNA-1_B23MHV2LT4_1.chckpts/659_dm4-T1-TRNA-1_B23MHV2LT4_1.gene_reads.sorted.sifted.bam.tmpsorted.ok
08:27:44 : INFO : Running: samtools index 659_dm4-T1-TRNA-1_B23MHV2LT4_1.gene_reads.sorted.sifted.bam
08:27:48 : INFO : Execution Time = 0.06 minutes. CMD: samtools index 659_dm4-T1-TRNA-1_B23MHV2LT4_1.gene_reads.sorted.sifted.bam
08:27:48 : INFO : Running: touch /tmp/nxf.j8dGJ2viId/659_dm4-T1-TRNA-1_B23MHV2LT4_1.chckpts/659_dm4-T1-TRNA-1_B23MHV2LT4_1.gene_reads.sorted.sifted.bam.indexed.ok
08:27:48 : INFO : Running: samtools index 659_dm4-T1-TRNA-1_B23MHV2LT4_1.cancer_intron_reads.sorted.bam
08:27:48 : INFO : Execution Time = 0.00 minutes. CMD: samtools index 659_dm4-T1-TRNA-1_B23MHV2LT4_1.cancer_intron_reads.sorted.bam
08:27:48 : INFO : Running: touch /tmp/nxf.j8dGJ2viId/659_dm4-T1-TRNA-1_B23MHV2LT4_1.chckpts/659_dm4-T1-TRNA-1_B23MHV2LT4_1.cancer_intron_reads.sorted.bam.indexed.ok
08:27:48 : INFO : Running: create_report 659_dm4-T1-TRNA-1_B23MHV2LT4_1.introns.for_IGV.bed ctat_genome_lib_build_dir/ref_genome.fa --type junction --output 659_dm4-T1-TRNA-1_B23MHV2LT4_1.ctat-splicing.igv.html --track-config 659_dm4-T1-TRNA-1_B23MHV2LT4_1.igv.tracks --info-columns gene variant_name uniquely_mapped multi_mapped TCGA GTEx --title 'CTAT_Splicing: 659_dm4-T1-TRNA-1_B23MHV2LT4_1'
08:32:28 : INFO : Execution Time = 4.67 minutes. CMD: create_report 659_dm4-T1-TRNA-1_B23MHV2LT4_1.introns.for_IGV.bed ctat_genome_lib_build_dir/ref_genome.fa --type junction --output 659_dm4-T1-TRNA-1_B23MHV2LT4_1.ctat-splicing.igv.html --track-config 659_dm4-T1-TRNA-1_B23MHV2LT4_1.igv.tracks --info-columns gene variant_name uniquely_mapped multi_mapped TCGA GTEx --title 'CTAT_Splicing: 659_dm4-T1-TRNA-1_B23MHV2LT4_1'
08:32:28 : INFO : Running: touch /tmp/nxf.j8dGJ2viId/659_dm4-T1-TRNA-1_B23MHV2LT4_1.chckpts/igv_create_html.ok
08:32:28 : INFO : done.