[W::hts_idx_load3] The index file is older than the data file: 659_bNS-T1-TRNA-1_B23MHV2LT4_1.Aligned.sortedByCoord.out.bam.bai
Reading reference bed file:  ref_annot.bed  ...  Done
Load BAM file ...  Done

===================================================================
Total splicing  Events:	338190399
Known Splicing Events:	335181015
Partial Novel Splicing Events:	1572326
Novel Splicing Events:	1311840
Filtered Splicing Events:	125218

Total splicing  Junctions:	666432
Known Splicing Junctions:	293108
Partial Novel Splicing Junctions:	186854
Novel Splicing Junctions:	186470

===================================================================
Create BED file ...
Create Interact file ...