.command.err - Workdir - DAQ:SENTIEON_BWAMEM (1029_01S-N1-BDNA-1_B23KGCJLT4_1;L007;0001)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/87/da55b3f0a6a75327a475406220c4e4/.command.err
Size: 71.5 KB
Task: DAQ:SENTIEON_BWAMEM (1029_01S-N1-BDNA-1_B23KGCJLT4_1;L007;0001)
Attempt
Content

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1029_01S-N1-BDNA-1_B23KGCJLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1618.457 CPU sec, 655.871 real sec
[M::process] read 681670 sequences (100000291 bp) in 0.907 CPU sec, 0.911 real sec...
[M::process] read 680608 sequences (100000295 bp) in 1.109 CPU sec, 1.115 real sec...
[M::process] read 679718 sequences (100000028 bp) in 1.109 CPU sec, 1.106 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282716, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 330, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (345.91, 163.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679344 sequences (100000072 bp) in 1.058 CPU sec, 1.059 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281067, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 341, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (357.10, 167.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282400, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 337, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (353.00, 166.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681670 reads in 200.093 CPU sec, 6.387 real sec
[M::mem_process_seqs] Processed 679718 reads in 132.239 CPU sec, 4.260 real sec
[M::mem_process_seqs] Processed 680608 reads in 143.615 CPU sec, 4.648 real sec
sorting block 0, #reads = 1081649 mem = 536879448 peak = 584073216
[M::process] read 678700 sequences (100000143 bp) in 1.502 CPU sec, 1.777 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281282, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 346, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (361.07, 168.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678432 sequences (100000110 bp) in 1.190 CPU sec, 1.214 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281490, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 353, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (367.89, 171.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677868 sequences (100000062 bp) in 1.050 CPU sec, 1.043 real sec...
[M::mem_process_seqs] Processed 679344 reads in 123.723 CPU sec, 3.937 real sec
sorting block 1, #reads = 1080796 mem = 536880621 peak = 884953088
[M::mem_process_seqs] Processed 678700 reads in 78.748 CPU sec, 2.492 real sec
sorting block 2, #reads = 1080489 mem = 536880018 peak = 972787712
[M::process] read 677400 sequences (100000069 bp) in 1.504 CPU sec, 1.619 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281524, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 357, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (371.51, 173.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677218 sequences (100000010 bp) in 1.154 CPU sec, 1.165 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281280, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 361, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (375.67, 174.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678432 reads in 125.035 CPU sec, 3.978 real sec
[M::mem_process_seqs] Processed 677868 reads in 79.674 CPU sec, 2.548 real sec
sorting block 3, #reads = 1080127 mem = 536876167 peak = 972787712
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280955, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 362, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (376.43, 174.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677120 sequences (100000277 bp) in 1.667 CPU sec, 1.756 real sec...
[M::process] read 677228 sequences (100000294 bp) in 1.120 CPU sec, 1.121 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279828, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 363, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (376.98, 174.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677400 reads in 126.444 CPU sec, 4.023 real sec
[M::mem_process_seqs] Processed 677218 reads in 79.763 CPU sec, 2.549 real sec
sorting block 4, #reads = 1078949 mem = 536880128 peak = 972787712
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280451, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 362, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (378.08, 175.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681086 sequences (100000283 bp) in 1.636 CPU sec, 1.770 real sec...
[M::process] read 679940 sequences (100000101 bp) in 1.150 CPU sec, 1.144 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280682, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 361, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (378.66, 178.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677120 reads in 127.046 CPU sec, 4.047 real sec
[M::mem_process_seqs] Processed 677228 reads in 79.725 CPU sec, 2.538 real sec
sorting block 5, #reads = 1078652 mem = 536877328 peak = 972787712
[M::process] read 679434 sequences (100000199 bp) in 1.817 CPU sec, 1.937 real sec...
[M::process] read 678776 sequences (100000061 bp) in 1.180 CPU sec, 1.184 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281801, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 345, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (359.63, 169.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679940 reads in 67.807 CPU sec, 2.155 real sec
sorting block 6, #reads = 1079545 mem = 536879150 peak = 972787712
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281423, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 337, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (353.56, 167.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281499, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 349, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (363.21, 169.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678576 sequences (100000035 bp) in 1.464 CPU sec, 1.470 real sec...
[M::mem_process_seqs] Processed 681086 reads in 193.842 CPU sec, 6.162 real sec
[M::mem_process_seqs] Processed 679434 reads in 91.957 CPU sec, 2.935 real sec
sorting block 7, #reads = 1080461 mem = 536879223 peak = 972787712
[M::process] read 678202 sequences (100000258 bp) in 1.836 CPU sec, 1.968 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281008, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 357, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (370.75, 172.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677656 sequences (100000237 bp) in 1.210 CPU sec, 1.210 real sec...
[M::mem_process_seqs] Processed 678576 reads in 69.864 CPU sec, 2.204 real sec
sorting block 8, #reads = 1080458 mem = 536876980 peak = 972787712
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281665, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 352, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (365.19, 169.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281467, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 361, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (374.97, 175.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678776 reads in 195.004 CPU sec, 6.208 real sec
[M::process] read 677584 sequences (100000171 bp) in 1.366 CPU sec, 1.379 real sec...
[M::mem_process_seqs] Processed 678202 reads in 93.223 CPU sec, 2.985 real sec
sorting block 9, #reads = 1081039 mem = 536877744 peak = 972787712
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280739, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 362, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (375.55, 174.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677578 sequences (100000227 bp) in 1.847 CPU sec, 2.013 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280228, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 361, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (375.87, 173.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677918 sequences (100000281 bp) in 1.190 CPU sec, 1.196 real sec...
[M::mem_process_seqs] Processed 677656 reads in 126.869 CPU sec, 4.041 real sec
sorting block 10, #reads = 1078449 mem = 536882272 peak = 972787712
[M::mem_process_seqs] Processed 677584 reads in 80.885 CPU sec, 2.570 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280925, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 360, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (375.05, 175.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678002 sequences (100000149 bp) in 1.993 CPU sec, 2.126 real sec...
[M::process] read 680804 sequences (100000279 bp) in 1.250 CPU sec, 1.250 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280686, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 358, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (375.06, 177.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677578 reads in 127.131 CPU sec, 4.039 real sec
sorting block 11, #reads = 1078339 mem = 536879643 peak = 972787712
[M::mem_process_seqs] Processed 677918 reads in 80.323 CPU sec, 2.554 real sec
sorting block 12, #reads = 1079153 mem = 536878550 peak = 972787712
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281000, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 358, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (375.49, 178.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679888 sequences (100000222 bp) in 1.938 CPU sec, 2.055 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282304, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 338, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (353.97, 167.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679176 sequences (100000166 bp) in 1.260 CPU sec, 1.254 real sec...
[M::mem_process_seqs] Processed 678002 reads in 126.891 CPU sec, 4.035 real sec
[M::mem_process_seqs] Processed 680804 reads in 80.270 CPU sec, 2.544 real sec
sorting block 13, #reads = 1078330 mem = 536879574 peak = 972787712
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281454, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 346, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (361.01, 169.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678438 sequences (100000086 bp) in 2.063 CPU sec, 2.220 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280886, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 351, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (365.76, 171.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678106 sequences (100000068 bp) in 1.280 CPU sec, 1.280 real sec...
[M::mem_process_seqs] Processed 679888 reads in 124.453 CPU sec, 3.950 real sec
[M::mem_process_seqs] Processed 679176 reads in 79.072 CPU sec, 2.512 real sec
sorting block 14, #reads = 1080578 mem = 536879642 peak = 972787712
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281141, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 354, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (366.97, 169.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677522 sequences (100000097 bp) in 2.121 CPU sec, 2.272 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281399, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 359, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (372.70, 172.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677384 sequences (100000004 bp) in 1.260 CPU sec, 1.263 real sec...
[M::mem_process_seqs] Processed 678438 reads in 124.103 CPU sec, 3.942 real sec
sorting block 15, #reads = 1079505 mem = 536879678 peak = 972787712
[M::mem_process_seqs] Processed 678106 reads in 79.376 CPU sec, 2.514 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280712, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 361, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (375.53, 174.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 16, #reads = 1080468 mem = 536878740 peak = 972787712
[M::process] read 677190 sequences (100000148 bp) in 2.138 CPU sec, 2.295 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280558, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 363, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (377.93, 175.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677820 sequences (100000258 bp) in 1.290 CPU sec, 1.290 real sec...
[M::mem_process_seqs] Processed 677522 reads in 127.190 CPU sec, 4.043 real sec
[M::mem_process_seqs] Processed 677384 reads in 82.331 CPU sec, 2.612 real sec
sorting block 17, #reads = 1078239 mem = 536878964 peak = 972787712
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280726, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 365, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (379.32, 176.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677882 sequences (100000291 bp) in 2.054 CPU sec, 2.183 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280552, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 356, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (373.95, 176.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677554 sequences (100000146 bp) in 1.280 CPU sec, 1.279 real sec...
[M::mem_process_seqs] Processed 677190 reads in 127.587 CPU sec, 4.059 real sec
[M::mem_process_seqs] Processed 677820 reads in 81.992 CPU sec, 2.600 real sec
sorting block 18, #reads = 1078575 mem = 536879880 peak = 972787712
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281362, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 358, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (376.78, 179.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680736 sequences (100000053 bp) in 2.063 CPU sec, 2.181 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280864, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 361, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039)
[M::mem_pestat] mean and std.dev: (380.35, 181.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1307)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679844 sequences (100000276 bp) in 1.280 CPU sec, 1.285 real sec...
[M::mem_process_seqs] Processed 677882 reads in 125.795 CPU sec, 3.995 real sec
sorting block 19, #reads = 1079153 mem = 536880418 peak = 972787712
[M::process] read 679026 sequences (100000179 bp) in 1.475 CPU sec, 1.481 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281305, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 337, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (354.37, 169.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680736 reads in 75.077 CPU sec, 2.607 real sec
[M::mem_process_seqs] Processed 677554 reads in 174.929 CPU sec, 5.772 real sec
sorting block 20, #reads = 1080041 mem = 536882592 peak = 972787712
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281779, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 346, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (362.37, 171.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678556 sequences (100000002 bp) in 2.055 CPU sec, 2.213 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281681, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 352, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (367.82, 173.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679844 reads in 138.717 CPU sec, 4.416 real sec
[M::mem_process_seqs] Processed 679026 reads in 81.455 CPU sec, 2.570 real sec
[M::process] read 677598 sequences (100000169 bp) in 1.379 CPU sec, 1.417 real sec...
sorting block 21, #reads = 1080330 mem = 536877773 peak = 972787712
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281322, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 353, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (368.82, 172.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677770 sequences (100000140 bp) in 1.944 CPU sec, 2.085 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280538, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 360, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (374.71, 173.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677310 sequences (100000008 bp) in 1.250 CPU sec, 1.248 real sec...
[M::mem_process_seqs] Processed 678556 reads in 126.712 CPU sec, 4.031 real sec
sorting block 22, #reads = 1080753 mem = 536877918 peak = 972787712
[M::mem_process_seqs] Processed 677598 reads in 80.549 CPU sec, 2.556 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280577, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 361, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (375.77, 175.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678044 sequences (100000270 bp) in 2.166 CPU sec, 2.308 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280538, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 360, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (375.56, 174.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677886 sequences (100000003 bp) in 1.250 CPU sec, 1.253 real sec...
[M::mem_process_seqs] Processed 677770 reads in 128.905 CPU sec, 4.096 real sec
sorting block 23, #reads = 1079400 mem = 536877536 peak = 972787712
[M::mem_process_seqs] Processed 677310 reads in 82.279 CPU sec, 2.609 real sec
sorting block 24, #reads = 1078812 mem = 536880847 peak = 1011683328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280478, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 355, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (371.93, 175.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677874 sequences (100000085 bp) in 1.965 CPU sec, 2.107 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280669, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 353, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (370.70, 174.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678054 sequences (100000211 bp) in 1.290 CPU sec, 1.288 real sec...
[M::mem_process_seqs] Processed 678044 reads in 129.201 CPU sec, 4.112 real sec
[M::mem_process_seqs] Processed 677886 reads in 82.079 CPU sec, 2.600 real sec
sorting block 25, #reads = 1079128 mem = 536877107 peak = 1011683328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281151, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 355, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (373.53, 177.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1280)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683336 sequences (100000003 bp) in 1.971 CPU sec, 2.106 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281124, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 358, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (376.47, 179.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681482 sequences (100000166 bp) in 1.310 CPU sec, 1.316 real sec...
[M::mem_process_seqs] Processed 677874 reads in 127.706 CPU sec, 4.066 real sec
[M::mem_process_seqs] Processed 678054 reads in 81.362 CPU sec, 2.573 real sec
sorting block 26, #reads = 1079354 mem = 536879012 peak = 1011683328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283796, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 324, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (344.93, 169.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680768 sequences (100000039 bp) in 2.090 CPU sec, 2.197 real sec...
[M::process] read 679962 sequences (100000297 bp) in 1.310 CPU sec, 1.319 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282568, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 336, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (353.07, 169.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683336 reads in 133.822 CPU sec, 4.256 real sec
sorting block 27, #reads = 1078585 mem = 536884024 peak = 1011683328
[M::mem_process_seqs] Processed 681482 reads in 83.107 CPU sec, 2.636 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282481, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 341, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (356.35, 168.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 28, #reads = 1080694 mem = 536878690 peak = 1021493248
[M::process] read 679828 sequences (100000059 bp) in 2.248 CPU sec, 2.387 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281649, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 346, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (360.75, 168.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679668 sequences (100000097 bp) in 1.290 CPU sec, 1.297 real sec...
[M::mem_process_seqs] Processed 680768 reads in 131.383 CPU sec, 4.178 real sec
[M::mem_process_seqs] Processed 679962 reads in 83.648 CPU sec, 2.651 real sec
sorting block 29, #reads = 1079669 mem = 536881035 peak = 1021493248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281986, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 348, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (362.11, 169.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679178 sequences (100000246 bp) in 2.170 CPU sec, 2.305 real sec...
[M::process] read 678936 sequences (100000052 bp) in 1.310 CPU sec, 1.306 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281923, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 350, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (363.50, 170.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679828 reads in 137.707 CPU sec, 4.382 real sec
[M::mem_process_seqs] Processed 679668 reads in 88.185 CPU sec, 2.798 real sec
sorting block 30, #reads = 1077662 mem = 536878210 peak = 1021493248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281772, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 342, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (356.91, 164.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678620 sequences (100000119 bp) in 1.993 CPU sec, 2.148 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282277, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 344, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (358.32, 165.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678328 sequences (100000157 bp) in 1.290 CPU sec, 1.291 real sec...
[M::mem_process_seqs] Processed 679178 reads in 136.810 CPU sec, 4.363 real sec
[M::mem_process_seqs] Processed 678936 reads in 84.457 CPU sec, 2.678 real sec
sorting block 31, #reads = 1076432 mem = 536883050 peak = 1021493248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281856, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 344, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (357.45, 163.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681024 sequences (100000189 bp) in 2.241 CPU sec, 2.364 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281192, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 347, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (361.28, 166.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681862 sequences (100000027 bp) in 1.350 CPU sec, 1.341 real sec...
[M::mem_process_seqs] Processed 678620 reads in 134.784 CPU sec, 4.282 real sec
sorting block 32, #reads = 1078951 mem = 536877244 peak = 1021493248
[M::mem_process_seqs] Processed 678328 reads in 85.415 CPU sec, 2.706 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283129, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 334, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (352.28, 168.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 33, #reads = 1077641 mem = 536879139 peak = 1041731584
[M::process] read 681274 sequences (100000286 bp) in 2.245 CPU sec, 2.390 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283250, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 328, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (345.71, 164.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680382 sequences (100000254 bp) in 1.300 CPU sec, 1.304 real sec...
[M::mem_process_seqs] Processed 681024 reads in 134.869 CPU sec, 4.287 real sec
[M::mem_process_seqs] Processed 681862 reads in 84.801 CPU sec, 2.686 real sec
sorting block 34, #reads = 1078516 mem = 536879451 peak = 1041731584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282575, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 334, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (349.49, 164.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679938 sequences (100000264 bp) in 2.042 CPU sec, 2.162 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282418, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 338, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (353.23, 165.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679752 sequences (100000212 bp) in 1.320 CPU sec, 1.319 real sec...
[M::mem_process_seqs] Processed 681274 reads in 134.710 CPU sec, 4.288 real sec
[M::mem_process_seqs] Processed 680382 reads in 84.737 CPU sec, 2.686 real sec
sorting block 35, #reads = 1078435 mem = 536885786 peak = 1041731584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281728, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 342, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (357.42, 167.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679264 sequences (100000126 bp) in 2.137 CPU sec, 2.249 real sec...
[M::process] read 678924 sequences (100000191 bp) in 1.280 CPU sec, 1.277 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282253, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 342, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (357.31, 166.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679938 reads in 134.825 CPU sec, 4.290 real sec
[M::mem_process_seqs] Processed 679752 reads in 85.531 CPU sec, 2.714 real sec
sorting block 36, #reads = 1078138 mem = 536878627 peak = 1041731584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281816, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 345, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (358.95, 166.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678398 sequences (100000123 bp) in 2.224 CPU sec, 2.345 real sec...
[M::process] read 678246 sequences (100000260 bp) in 1.308 CPU sec, 1.304 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282494, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 346, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (360.37, 166.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679264 reads in 148.094 CPU sec, 4.717 real sec
sorting block 37, #reads = 1077516 mem = 536880824 peak = 1041731584
[M::mem_process_seqs] Processed 678924 reads in 93.061 CPU sec, 2.962 real sec
sorting block 38, #reads = 1074279 mem = 536876664 peak = 1041731584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281945, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 352, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (366.19, 169.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679192 sequences (100000050 bp) in 2.242 CPU sec, 2.375 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281906, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 354, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (369.42, 173.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681904 sequences (100000141 bp) in 1.300 CPU sec, 1.298 real sec...
[M::mem_process_seqs] Processed 678398 reads in 133.878 CPU sec, 4.254 real sec
[M::mem_process_seqs] Processed 678246 reads in 83.940 CPU sec, 2.664 real sec
sorting block 39, #reads = 1075840 mem = 536878186 peak = 1041731584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282155, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 349, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (367.20, 174.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680680 sequences (100000176 bp) in 2.159 CPU sec, 2.260 real sec...
[M::process] read 680222 sequences (100000194 bp) in 1.320 CPU sec, 1.323 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283103, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 329, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (346.36, 165.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679192 reads in 142.366 CPU sec, 4.531 real sec
[M::mem_process_seqs] Processed 681904 reads in 89.158 CPU sec, 2.834 real sec
sorting block 40, #reads = 1075592 mem = 536879523 peak = 1041731584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282770, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 334, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (350.92, 165.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679752 sequences (100000143 bp) in 2.133 CPU sec, 2.246 real sec...
[M::process] read 679444 sequences (100000274 bp) in 1.280 CPU sec, 1.279 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282136, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 339, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (354.39, 166.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680680 reads in 136.162 CPU sec, 4.334 real sec
sorting block 41, #reads = 1078629 mem = 536881095 peak = 1041731584
[M::mem_process_seqs] Processed 680222 reads in 86.689 CPU sec, 2.753 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282562, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 341, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (356.00, 166.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678924 sequences (100000121 bp) in 2.234 CPU sec, 2.348 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282190, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 344, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (358.20, 165.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678214 sequences (100000081 bp) in 1.290 CPU sec, 1.292 real sec...
[M::mem_process_seqs] Processed 679752 reads in 132.736 CPU sec, 4.220 real sec
sorting block 42, #reads = 1078329 mem = 536879458 peak = 1068498944
[M::mem_process_seqs] Processed 679444 reads in 85.544 CPU sec, 2.715 real sec
sorting block 43, #reads = 1077914 mem = 536879889 peak = 1076117504
[M::process] read 677906 sequences (100000053 bp) in 2.144 CPU sec, 2.255 real sec...
[M::process] read 677864 sequences (100000289 bp) in 1.310 CPU sec, 1.305 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281947, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 352, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (367.04, 170.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678214 reads in 77.395 CPU sec, 2.452 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282318, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 348, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (362.51, 168.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281347, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 354, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (369.51, 171.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678088 sequences (100000248 bp) in 1.541 CPU sec, 1.545 real sec...
[M::mem_process_seqs] Processed 678924 reads in 214.506 CPU sec, 6.827 real sec
sorting block 44, #reads = 1076977 mem = 536878638 peak = 1076117504
[M::mem_process_seqs] Processed 677906 reads in 106.690 CPU sec, 3.414 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281556, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 353, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (369.54, 172.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 45, #reads = 1074661 mem = 536878915 peak = 1076117504
[M::process] read 681246 sequences (100000012 bp) in 2.464 CPU sec, 2.630 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282047, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 353, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (370.46, 175.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681556 sequences (100000061 bp) in 1.310 CPU sec, 1.307 real sec...
[M::mem_process_seqs] Processed 677864 reads in 143.299 CPU sec, 4.556 real sec
[M::mem_process_seqs] Processed 678088 reads in 89.273 CPU sec, 2.834 real sec
sorting block 46, #reads = 1073288 mem = 536881672 peak = 1076117504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282244, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 335, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (354.35, 171.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680500 sequences (100000098 bp) in 1.971 CPU sec, 2.140 real sec...
[M::process] read 679978 sequences (100000075 bp) in 1.420 CPU sec, 1.412 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282669, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 333, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (350.58, 167.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681246 reads in 141.158 CPU sec, 4.487 real sec
[M::mem_process_seqs] Processed 681556 reads in 88.644 CPU sec, 2.812 real sec
sorting block 47, #reads = 1074861 mem = 536878581 peak = 1076117504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282698, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 343, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (357.90, 169.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679318 sequences (100000083 bp) in 2.069 CPU sec, 2.170 real sec...
[M::process] read 678482 sequences (100000044 bp) in 1.310 CPU sec, 1.310 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282138, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 346, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (361.47, 170.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680500 reads in 131.936 CPU sec, 4.193 real sec
sorting block 48, #reads = 1077595 mem = 536880456 peak = 1076117504
[M::mem_process_seqs] Processed 679978 reads in 83.898 CPU sec, 2.657 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282133, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 355, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (367.96, 171.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678280 sequences (100000235 bp) in 1.792 CPU sec, 1.910 real sec...
[M::process] read 678506 sequences (100000237 bp) in 1.400 CPU sec, 1.397 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281868, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 356, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (369.67, 171.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679318 reads in 149.396 CPU sec, 4.759 real sec
sorting block 49, #reads = 1077687 mem = 536880263 peak = 1098489856
[M::mem_process_seqs] Processed 678482 reads in 103.477 CPU sec, 3.293 real sec
sorting block 50, #reads = 1075145 mem = 536879136 peak = 1098489856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281611, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 356, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (369.98, 172.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678250 sequences (100000272 bp) in 2.517 CPU sec, 2.664 real sec...
[M::process] read 678116 sequences (100000124 bp) in 1.390 CPU sec, 1.385 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281976, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 349, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (363.91, 168.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678280 reads in 156.690 CPU sec, 4.975 real sec
[M::mem_process_seqs] Processed 678506 reads in 98.806 CPU sec, 3.132 real sec
sorting block 51, #reads = 1074810 mem = 536880014 peak = 1098489856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280942, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 349, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (365.27, 170.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 533386 sequences (78681582 bp) in 1.901 CPU sec, 2.017 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281020, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 349, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (365.86, 171.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678250 reads in 154.780 CPU sec, 4.979 real sec
[M::mem_process_seqs] Processed 678116 reads in 96.222 CPU sec, 3.116 real sec
sorting block 52, #reads = 1075202 mem = 536876559 peak = 1098489856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 221416, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 353, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (370.55, 174.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 533386 reads in 65.858 CPU sec, 2.184 real sec
sorting block 53, #reads = 1075944 mem = 536878482 peak = 1098489856
os version: Linux 6.12.83
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 58253498
overall: 69831659520 mem 7589.238 user 286.200 sys 863.939 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:B23KGCJLT4.1029_01S-N1-BDNA-1_B23KGCJLT4_1.L007\tPU:L007\tSM:1029_01S-N1-BDNA-1_B23KGCJLT4_1\tLB:1029_01S-N1-BDNA-1_B23KGCJLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1029_01S-N1-BDNA-1_B23KGCJLT4_1_R1.fastq.gz 1029_01S-N1-BDNA-1_B23KGCJLT4_1_R2.fastq.gz
[main] Real time: 863.975 sec; CPU: 7875.442 sec
sorting block 54, #reads = 281326 mem = 140424104 peak = 1098489856
uniq header count 1
merging 55 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.83
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 3081253061
output reads: 58511090
bam_mem_sort: 55 calls 23.048 user 0.282 sys 23.437 real
bam_write: 55 calls 35.640 user 0.944 sys 36.867 real
execute: 1 calls 15.794 user 0.859 sys 882.640 real
merge_files: 1 calls 15.775 user 0.839 sys 18.527 real
parse_chunk: 869 calls 27.683 user 7.158 sys 35.433 real
read_chunk: 2343 calls 21.984 user 19.707 sys 208.693 real
sort_block: 1 calls 17.099 user 19.134 sys 200.922 real
write_chunk: 1472 calls 15.387 user 0.686 sys 17.818 real
overall: 2556559360 mem 685.695 user 36.354 sys 882.660 real
ls: cannot access '*.cram': No such file or directory