.command.err - Workdir - DAQ:SENTIEON_BWAMEM (1029_01S-N1-BDNA-1_B23KGCJLT4_1;L001;0001)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/bb/2416c218adef328fbf996b1367cff2/.command.err
Size: 74.6 KB
Task: DAQ:SENTIEON_BWAMEM (1029_01S-N1-BDNA-1_B23KGCJLT4_1;L001;0001)
Attempt
Content

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1029_01S-N1-BDNA-1_B23KGCJLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1542.661 CPU sec, 530.602 real sec
[M::process] read 679554 sequences (100000155 bp) in 0.882 CPU sec, 0.884 real sec...
[M::process] read 678880 sequences (100000067 bp) in 1.088 CPU sec, 1.121 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281553, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 351, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (365.07, 170.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679188 sequences (100000082 bp) in 1.059 CPU sec, 1.066 real sec...
[M::process] read 679124 sequences (100000098 bp) in 1.070 CPU sec, 1.065 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281397, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 355, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (367.54, 169.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679554 reads in 130.660 CPU sec, 4.151 real sec
[M::mem_process_seqs] Processed 678880 reads in 75.988 CPU sec, 2.427 real sec
sorting block 0, #reads = 1079883 mem = 536882608 peak = 613310464
[M::process] read 678536 sequences (100000166 bp) in 1.435 CPU sec, 1.514 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281695, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 348, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (362.70, 169.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677914 sequences (100000133 bp) in 1.110 CPU sec, 1.107 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281785, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 358, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (373.36, 176.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281635, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 360, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (375.98, 177.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1294)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679188 reads in 172.105 CPU sec, 5.495 real sec
[M::mem_process_seqs] Processed 679124 reads in 128.166 CPU sec, 4.113 real sec
[M::mem_process_seqs] Processed 678536 reads in 140.063 CPU sec, 4.513 real sec
sorting block 1, #reads = 1079722 mem = 536878871 peak = 742285312
[M::process] read 677352 sequences (100000001 bp) in 1.749 CPU sec, 1.850 real sec...
sorting block 2, #reads = 1080288 mem = 536879020 peak = 858193920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280804, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 363, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (377.07, 175.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677416 sequences (100000041 bp) in 1.363 CPU sec, 1.388 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280989, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 366, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (379.90, 176.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677914 reads in 125.319 CPU sec, 3.982 real sec
[M::mem_process_seqs] Processed 677352 reads in 79.926 CPU sec, 2.518 real sec
[M::process] read 677696 sequences (100000084 bp) in 1.343 CPU sec, 1.383 real sec...
sorting block 3, #reads = 1079429 mem = 536879069 peak = 858193920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280098, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 363, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (377.50, 174.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677462 sequences (100000011 bp) in 1.699 CPU sec, 1.779 real sec...
[M::process] read 677418 sequences (100000100 bp) in 1.270 CPU sec, 1.267 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280138, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 359, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (375.30, 176.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677416 reads in 125.709 CPU sec, 3.997 real sec
[M::mem_process_seqs] Processed 677696 reads in 79.275 CPU sec, 2.513 real sec
sorting block 4, #reads = 1079151 mem = 536878303 peak = 858193920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280599, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 360, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (378.01, 178.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678720 sequences (100000223 bp) in 1.607 CPU sec, 1.687 real sec...
[M::process] read 677824 sequences (100000058 bp) in 1.193 CPU sec, 1.199 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281129, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 359, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (377.97, 179.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677462 reads in 123.536 CPU sec, 3.933 real sec
sorting block 5, #reads = 1078777 mem = 536877306 peak = 858193920
[M::mem_process_seqs] Processed 677418 reads in 77.605 CPU sec, 2.473 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281466, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 358, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (371.59, 173.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678724 sequences (100000270 bp) in 1.944 CPU sec, 2.059 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281287, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 364, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (375.81, 172.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678554 sequences (100000258 bp) in 1.240 CPU sec, 1.240 real sec...
[M::mem_process_seqs] Processed 678720 reads in 118.749 CPU sec, 3.773 real sec
sorting block 6, #reads = 1079421 mem = 536880098 peak = 858193920
[M::mem_process_seqs] Processed 677824 reads in 74.569 CPU sec, 2.360 real sec
sorting block 7, #reads = 1080347 mem = 536880776 peak = 936321024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281824, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 357, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (370.85, 172.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678274 sequences (100000068 bp) in 1.668 CPU sec, 1.797 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281678, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 363, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (377.00, 176.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677904 sequences (100000034 bp) in 1.280 CPU sec, 1.282 real sec...
[M::mem_process_seqs] Processed 678724 reads in 120.203 CPU sec, 3.827 real sec
[M::mem_process_seqs] Processed 678554 reads in 76.986 CPU sec, 2.440 real sec
sorting block 8, #reads = 1080335 mem = 536879027 peak = 936321024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281180, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 366, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (379.37, 177.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1297)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677438 sequences (100000123 bp) in 1.962 CPU sec, 2.089 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281490, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 368, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (381.46, 177.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677676 sequences (100000132 bp) in 1.320 CPU sec, 1.316 real sec...
[M::mem_process_seqs] Processed 678274 reads in 122.364 CPU sec, 3.884 real sec
[M::mem_process_seqs] Processed 677904 reads in 77.478 CPU sec, 2.454 real sec
sorting block 9, #reads = 1080280 mem = 536880799 peak = 936321024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281020, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 369, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (382.19, 177.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677836 sequences (100000132 bp) in 1.883 CPU sec, 1.974 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281084, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 362, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (377.12, 175.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677720 sequences (100000186 bp) in 1.300 CPU sec, 1.306 real sec...
[M::mem_process_seqs] Processed 677438 reads in 125.177 CPU sec, 3.981 real sec
sorting block 10, #reads = 1080651 mem = 536879264 peak = 936321024
[M::mem_process_seqs] Processed 677676 reads in 79.901 CPU sec, 2.529 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281029, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 362, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (378.35, 178.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1292)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677420 sequences (100000128 bp) in 2.101 CPU sec, 2.227 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280969, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 365, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044)
[M::mem_pestat] mean and std.dev: (381.53, 180.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1313)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678596 sequences (100000144 bp) in 1.340 CPU sec, 1.344 real sec...
[M::mem_process_seqs] Processed 677836 reads in 124.189 CPU sec, 3.941 real sec
sorting block 11, #reads = 1078585 mem = 536882152 peak = 936321024
[M::mem_process_seqs] Processed 677720 reads in 78.858 CPU sec, 2.498 real sec
sorting block 12, #reads = 1078341 mem = 536887455 peak = 960868352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280875, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 365, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (381.18, 179.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1302)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678190 sequences (100000143 bp) in 1.804 CPU sec, 1.917 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282039, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 363, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (377.35, 178.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1291)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678316 sequences (100000216 bp) in 1.330 CPU sec, 1.335 real sec...
[M::mem_process_seqs] Processed 677420 reads in 123.223 CPU sec, 3.923 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281392, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 365, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (377.28, 175.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678492 sequences (100000188 bp) in 1.474 CPU sec, 1.480 real sec...
[M::mem_process_seqs] Processed 678190 reads in 69.432 CPU sec, 2.397 real sec
sorting block 13, #reads = 1079266 mem = 536880094 peak = 960868352
[M::mem_process_seqs] Processed 678596 reads in 169.707 CPU sec, 5.583 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281824, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 362, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (376.07, 176.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 14, #reads = 1079826 mem = 536879340 peak = 960868352
[M::process] read 678216 sequences (100000023 bp) in 1.929 CPU sec, 2.039 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281274, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 360, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (374.60, 175.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678316 reads in 131.774 CPU sec, 4.191 real sec
[M::mem_process_seqs] Processed 678492 reads in 78.937 CPU sec, 2.488 real sec
[M::process] read 677692 sequences (100000291 bp) in 1.661 CPU sec, 1.725 real sec...
sorting block 15, #reads = 1080437 mem = 536879640 peak = 960868352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281394, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 366, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (380.25, 179.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1301)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677488 sequences (100000096 bp) in 1.778 CPU sec, 1.898 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281552, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 369, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (383.91, 180.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1308)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678216 reads in 122.666 CPU sec, 3.913 real sec
[M::process] read 677416 sequences (100000110 bp) in 1.328 CPU sec, 1.329 real sec...
[M::mem_process_seqs] Processed 677692 reads in 79.161 CPU sec, 2.502 real sec
sorting block 16, #reads = 1080735 mem = 536878687 peak = 960868352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281214, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 368, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (382.68, 179.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677936 sequences (100000074 bp) in 1.993 CPU sec, 2.106 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281001, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 368, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040)
[M::mem_pestat] mean and std.dev: (383.47, 180.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1307)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677488 reads in 121.185 CPU sec, 3.850 real sec
[M::process] read 677766 sequences (100000221 bp) in 1.319 CPU sec, 1.323 real sec...
[M::mem_process_seqs] Processed 677416 reads in 77.710 CPU sec, 2.450 real sec
sorting block 17, #reads = 1080072 mem = 536881530 peak = 960868352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280974, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 362, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (379.73, 181.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1300)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677100 sequences (100000175 bp) in 2.078 CPU sec, 2.187 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280763, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 362, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (380.03, 181.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1300)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677882 sequences (100000284 bp) in 1.320 CPU sec, 1.317 real sec...
[M::mem_process_seqs] Processed 677936 reads in 119.810 CPU sec, 3.802 real sec
sorting block 18, #reads = 1080211 mem = 536878121 peak = 960868352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280527, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 369, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039)
[M::mem_pestat] mean and std.dev: (384.93, 181.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1305)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678710 sequences (100000243 bp) in 1.376 CPU sec, 1.380 real sec...
[M::mem_process_seqs] Processed 677100 reads in 69.799 CPU sec, 2.402 real sec
sorting block 19, #reads = 1078519 mem = 536878871 peak = 960868352
[M::mem_process_seqs] Processed 677766 reads in 164.300 CPU sec, 5.400 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281135, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 364, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (380.79, 180.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1301)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678564 sequences (100000047 bp) in 2.032 CPU sec, 2.182 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280986, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 362, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (378.07, 180.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1302)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677882 reads in 129.048 CPU sec, 4.099 real sec
[M::mem_process_seqs] Processed 678710 reads in 75.757 CPU sec, 2.389 real sec
sorting block 20, #reads = 1079636 mem = 536879744 peak = 960868352
[M::process] read 678766 sequences (100000291 bp) in 1.663 CPU sec, 1.761 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281521, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 360, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (374.65, 176.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 21, #reads = 1079680 mem = 536882444 peak = 960868352
[M::process] read 678700 sequences (100000153 bp) in 1.797 CPU sec, 1.925 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281005, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 353, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (368.84, 174.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678564 reads in 118.718 CPU sec, 3.778 real sec
[M::mem_process_seqs] Processed 678766 reads in 77.994 CPU sec, 2.453 real sec
[M::process] read 678148 sequences (100000011 bp) in 1.566 CPU sec, 1.613 real sec...
sorting block 22, #reads = 1080993 mem = 536878795 peak = 960868352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281035, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 356, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (372.95, 177.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677914 sequences (100000200 bp) in 1.891 CPU sec, 2.016 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281611, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 360, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040)
[M::mem_pestat] mean and std.dev: (378.04, 180.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1309)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678700 reads in 121.881 CPU sec, 3.881 real sec
[M::process] read 677682 sequences (100000199 bp) in 1.457 CPU sec, 1.482 real sec...
[M::mem_process_seqs] Processed 678148 reads in 79.159 CPU sec, 2.494 real sec
sorting block 23, #reads = 1080241 mem = 536878526 peak = 960868352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279715, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 358, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (376.03, 177.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678044 sequences (100000200 bp) in 1.814 CPU sec, 1.915 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280645, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 360, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (376.70, 177.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677508 sequences (100000106 bp) in 1.310 CPU sec, 1.307 real sec...
[M::mem_process_seqs] Processed 677914 reads in 122.602 CPU sec, 3.900 real sec
[M::mem_process_seqs] Processed 677682 reads in 77.784 CPU sec, 2.456 real sec
sorting block 24, #reads = 1078592 mem = 536877728 peak = 960868352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280809, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 357, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (375.03, 178.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677360 sequences (100000154 bp) in 2.187 CPU sec, 2.289 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280425, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 359, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (376.51, 177.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677956 sequences (100000134 bp) in 1.300 CPU sec, 1.303 real sec...
[M::mem_process_seqs] Processed 678044 reads in 122.438 CPU sec, 3.879 real sec
sorting block 25, #reads = 1078903 mem = 536879919 peak = 960868352
[M::mem_process_seqs] Processed 677508 reads in 78.545 CPU sec, 2.479 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281004, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 364, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (381.74, 181.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1309)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 26, #reads = 1078779 mem = 536880130 peak = 971513856
[M::process] read 680142 sequences (100000015 bp) in 1.807 CPU sec, 1.920 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281449, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 359, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (377.27, 180.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1294)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679732 sequences (100000184 bp) in 1.300 CPU sec, 1.304 real sec...
[M::mem_process_seqs] Processed 677360 reads in 120.817 CPU sec, 3.838 real sec
[M::mem_process_seqs] Processed 677956 reads in 77.333 CPU sec, 2.443 real sec
sorting block 27, #reads = 1078295 mem = 536878962 peak = 971513856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281958, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 351, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (365.77, 174.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680168 sequences (100000270 bp) in 2.109 CPU sec, 2.208 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281237, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 346, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (360.90, 170.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679874 sequences (100000266 bp) in 1.330 CPU sec, 1.335 real sec...
[M::mem_process_seqs] Processed 680142 reads in 120.893 CPU sec, 3.831 real sec
[M::mem_process_seqs] Processed 679732 reads in 77.139 CPU sec, 2.434 real sec
sorting block 28, #reads = 1082029 mem = 536878795 peak = 971513856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281673, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 342, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (357.40, 168.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679596 sequences (100000064 bp) in 1.866 CPU sec, 1.985 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281755, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 344, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (358.77, 168.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679376 sequences (100000190 bp) in 1.320 CPU sec, 1.321 real sec...
[M::mem_process_seqs] Processed 680168 reads in 122.721 CPU sec, 3.900 real sec
[M::mem_process_seqs] Processed 679874 reads in 77.511 CPU sec, 2.450 real sec
sorting block 29, #reads = 1081366 mem = 536879368 peak = 971513856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281349, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 350, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (364.65, 172.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679250 sequences (100000188 bp) in 1.916 CPU sec, 2.018 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281030, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 339, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (354.63, 164.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678964 sequences (100000097 bp) in 1.330 CPU sec, 1.327 real sec...
[M::mem_process_seqs] Processed 679596 reads in 125.116 CPU sec, 3.974 real sec
sorting block 30, #reads = 1081170 mem = 536877489 peak = 971513856
[M::mem_process_seqs] Processed 679376 reads in 79.439 CPU sec, 2.509 real sec
sorting block 31, #reads = 1079658 mem = 536880291 peak = 1019604992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281467, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 341, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (355.97, 165.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678574 sequences (100000011 bp) in 1.904 CPU sec, 2.006 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281271, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 342, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (357.83, 166.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677978 sequences (100000250 bp) in 1.340 CPU sec, 1.334 real sec...
[M::mem_process_seqs] Processed 679250 reads in 125.255 CPU sec, 3.980 real sec
[M::mem_process_seqs] Processed 678964 reads in 79.146 CPU sec, 2.500 real sec
sorting block 32, #reads = 1080177 mem = 536880183 peak = 1019604992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280959, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 343, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (358.35, 164.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678106 sequences (100000099 bp) in 2.187 CPU sec, 2.292 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281084, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 347, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (363.87, 168.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680098 sequences (100000069 bp) in 1.300 CPU sec, 1.300 real sec...
[M::mem_process_seqs] Processed 678574 reads in 124.599 CPU sec, 3.957 real sec
[M::mem_process_seqs] Processed 677978 reads in 79.538 CPU sec, 2.522 real sec
sorting block 33, #reads = 1079827 mem = 536880068 peak = 1019604992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280630, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 352, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (370.61, 175.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679180 sequences (100000174 bp) in 1.921 CPU sec, 2.037 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281617, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 347, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (361.77, 171.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680006 sequences (100000243 bp) in 1.300 CPU sec, 1.292 real sec...
[M::mem_process_seqs] Processed 678106 reads in 122.754 CPU sec, 3.904 real sec
sorting block 34, #reads = 1078451 mem = 536878657 peak = 1019604992
[M::mem_process_seqs] Processed 680098 reads in 76.456 CPU sec, 2.416 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282192, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 345, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (359.74, 167.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680106 sequences (100000175 bp) in 2.229 CPU sec, 2.328 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282171, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 335, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (351.51, 165.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679180 reads in 117.891 CPU sec, 3.744 real sec
sorting block 35, #reads = 1080396 mem = 536879492 peak = 1019604992
[M::mem_process_seqs] Processed 680006 reads in 76.177 CPU sec, 2.396 real sec
[M::process] read 679320 sequences (100000256 bp) in 1.480 CPU sec, 1.510 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281960, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 334, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (349.82, 163.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 36, #reads = 1080947 mem = 536877253 peak = 1019604992
[M::process] read 679194 sequences (100000182 bp) in 1.905 CPU sec, 2.012 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281545, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 341, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (357.06, 167.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680106 reads in 121.825 CPU sec, 3.899 real sec
[M::mem_process_seqs] Processed 679320 reads in 77.381 CPU sec, 2.443 real sec
[M::process] read 679002 sequences (100000143 bp) in 1.647 CPU sec, 1.691 real sec...
sorting block 37, #reads = 1081714 mem = 536879921 peak = 1019604992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281437, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 343, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (359.39, 167.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678436 sequences (100000009 bp) in 2.013 CPU sec, 2.131 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281048, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 344, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (358.90, 166.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679194 reads in 123.530 CPU sec, 3.926 real sec
[M::process] read 678132 sequences (100000125 bp) in 1.349 CPU sec, 1.366 real sec...
[M::mem_process_seqs] Processed 679002 reads in 79.428 CPU sec, 2.504 real sec
sorting block 38, #reads = 1080839 mem = 536881080 peak = 1019604992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281085, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 349, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (364.02, 169.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677658 sequences (100000075 bp) in 1.915 CPU sec, 2.050 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281112, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 354, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (369.84, 172.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678436 reads in 121.210 CPU sec, 3.855 real sec
[M::process] read 677720 sequences (100000057 bp) in 1.375 CPU sec, 1.380 real sec...
sorting block 39, #reads = 1079176 mem = 536877918 peak = 1019604992
[M::mem_process_seqs] Processed 678132 reads in 76.998 CPU sec, 2.426 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281018, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 354, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (370.57, 173.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 40, #reads = 1080041 mem = 536878380 peak = 1019604992
[M::process] read 680104 sequences (100000297 bp) in 2.073 CPU sec, 2.181 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281240, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 358, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (376.42, 179.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678852 sequences (100000006 bp) in 1.290 CPU sec, 1.284 real sec...
[M::mem_process_seqs] Processed 677658 reads in 125.228 CPU sec, 3.974 real sec
[M::mem_process_seqs] Processed 677720 reads in 81.736 CPU sec, 2.578 real sec
sorting block 41, #reads = 1078723 mem = 536882453 peak = 1019604992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282288, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 347, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (363.83, 174.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679830 sequences (100000116 bp) in 2.116 CPU sec, 2.233 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281306, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 352, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (367.33, 173.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680104 reads in 120.408 CPU sec, 3.824 real sec
[M::mem_process_seqs] Processed 678852 reads in 75.643 CPU sec, 2.376 real sec
[M::process] read 679696 sequences (100000159 bp) in 1.917 CPU sec, 1.992 real sec...
sorting block 42, #reads = 1077737 mem = 536881038 peak = 1019604992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282206, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 341, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (357.93, 169.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679132 sequences (100000205 bp) in 1.924 CPU sec, 2.039 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282003, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 342, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (358.39, 169.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679830 reads in 117.294 CPU sec, 3.731 real sec
[M::mem_process_seqs] Processed 679696 reads in 75.744 CPU sec, 2.383 real sec
[M::process] read 678900 sequences (100000263 bp) in 1.636 CPU sec, 1.685 real sec...
sorting block 43, #reads = 1081201 mem = 536878913 peak = 1019604992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281932, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 344, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (360.88, 170.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678714 sequences (100000099 bp) in 2.000 CPU sec, 2.095 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282031, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 344, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (360.66, 169.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679132 reads in 116.069 CPU sec, 3.701 real sec
[M::mem_process_seqs] Processed 678900 reads in 74.357 CPU sec, 2.344 real sec
sorting block 44, #reads = 1080304 mem = 536878050 peak = 1019604992
[M::process] read 678286 sequences (100000218 bp) in 1.719 CPU sec, 1.825 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281638, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 345, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (361.98, 169.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 45, #reads = 1081430 mem = 536879812 peak = 1019604992
[M::process] read 677836 sequences (100000204 bp) in 1.849 CPU sec, 1.955 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281855, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 347, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (363.44, 169.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678714 reads in 118.722 CPU sec, 3.778 real sec
[M::mem_process_seqs] Processed 678286 reads in 76.281 CPU sec, 2.404 real sec
[M::process] read 677808 sequences (100000281 bp) in 1.755 CPU sec, 1.805 real sec...
sorting block 46, #reads = 1080514 mem = 536879657 peak = 1019604992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281480, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 353, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (368.08, 170.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677798 sequences (100000055 bp) in 1.786 CPU sec, 1.905 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282059, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 358, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (374.54, 175.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677836 reads in 123.398 CPU sec, 3.925 real sec
[M::process] read 680258 sequences (100000186 bp) in 1.374 CPU sec, 1.391 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281402, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 358, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (375.76, 178.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679546 sequences (100000012 bp) in 1.594 CPU sec, 1.596 real sec...
[M::mem_process_seqs] Processed 677798 reads in 70.183 CPU sec, 2.220 real sec
sorting block 47, #reads = 1078684 mem = 536878180 peak = 1019604992
[M::mem_process_seqs] Processed 677808 reads in 177.163 CPU sec, 5.612 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282493, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 350, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042)
[M::mem_pestat] mean and std.dev: (370.97, 183.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1315)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 48, #reads = 1077063 mem = 536879947 peak = 1070194688
[M::process] read 680220 sequences (100000144 bp) in 1.908 CPU sec, 2.033 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281746, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 357, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (374.21, 180.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1315)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680258 reads in 136.888 CPU sec, 4.344 real sec
[M::mem_process_seqs] Processed 679546 reads in 79.442 CPU sec, 2.503 real sec
[M::process] read 679522 sequences (100000169 bp) in 1.525 CPU sec, 1.565 real sec...
sorting block 49, #reads = 1079285 mem = 536876122 peak = 1070194688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282162, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 347, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (364.28, 175.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678956 sequences (100000105 bp) in 1.836 CPU sec, 1.919 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281960, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 347, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (363.98, 173.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678548 sequences (100000052 bp) in 1.260 CPU sec, 1.261 real sec...
[M::mem_process_seqs] Processed 680220 reads in 120.517 CPU sec, 3.822 real sec
[M::mem_process_seqs] Processed 679522 reads in 75.929 CPU sec, 2.402 real sec
sorting block 50, #reads = 1080868 mem = 536880772 peak = 1070194688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281762, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 353, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (369.82, 175.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678254 sequences (100000274 bp) in 1.847 CPU sec, 1.955 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280992, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 353, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (369.46, 173.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678956 reads in 120.129 CPU sec, 3.825 real sec
[M::process] read 678210 sequences (100000043 bp) in 1.316 CPU sec, 1.329 real sec...
sorting block 51, #reads = 1080648 mem = 536878147 peak = 1070194688
[M::mem_process_seqs] Processed 678548 reads in 77.299 CPU sec, 2.439 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281186, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 355, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (371.48, 174.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 52, #reads = 1079849 mem = 536877800 peak = 1070194688
[M::process] read 677634 sequences (100000087 bp) in 2.199 CPU sec, 2.295 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281800, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 351, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (367.97, 171.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677308 sequences (100000187 bp) in 1.300 CPU sec, 1.297 real sec...
[M::mem_process_seqs] Processed 678254 reads in 123.685 CPU sec, 3.919 real sec
[M::mem_process_seqs] Processed 678210 reads in 78.600 CPU sec, 2.480 real sec
sorting block 53, #reads = 1079136 mem = 536882223 peak = 1070194688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280888, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 352, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (369.12, 171.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677326 sequences (100000153 bp) in 1.849 CPU sec, 1.957 real sec...
[M::process] read 166434 sequences (24554725 bp) in 0.580 CPU sec, 0.572 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281156, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 355, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (372.98, 175.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677634 reads in 122.854 CPU sec, 3.926 real sec
[M::mem_process_seqs] Processed 677308 reads in 78.116 CPU sec, 2.493 real sec
sorting block 54, #reads = 1079781 mem = 536882186 peak = 1070194688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280692, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 358, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (376.15, 177.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 68951, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 357, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (376.46, 179.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1294)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677326 reads in 79.711 CPU sec, 2.587 real sec
[M::mem_process_seqs] Processed 166434 reads in 28.189 CPU sec, 0.909 real sec
sorting block 55, #reads = 1078062 mem = 536880529 peak = 1070194688
os version: Linux 6.12.83
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 60552856
overall: 69811015680 mem 7286.293 user 220.654 sys 727.513 real
sorting block 56, #reads = 351261 mem = 175235416 peak = 1070194688
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:B23KGCJLT4.1029_01S-N1-BDNA-1_B23KGCJLT4_1.L001\tPU:L001\tSM:1029_01S-N1-BDNA-1_B23KGCJLT4_1\tLB:1029_01S-N1-BDNA-1_B23KGCJLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1029_01S-N1-BDNA-1_B23KGCJLT4_1_R1.fastq.gz 1029_01S-N1-BDNA-1_B23KGCJLT4_1_R2.fastq.gz
[main] Real time: 727.562 sec; CPU: 7506.959 sec
uniq header count 1
merging 57 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.83
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 2945656763
output reads: 60819732
bam_mem_sort: 57 calls 29.182 user 0.156 sys 29.444 real
bam_write: 57 calls 11.675 user 0.617 sys 12.199 real
execute: 1 calls 11.535 user 0.564 sys 743.121 real
merge_files: 1 calls 11.514 user 0.540 sys 15.160 real
parse_chunk: 902 calls 38.149 user 9.628 sys 48.489 real
read_chunk: 2431 calls 16.529 user 16.369 sys 198.975 real
sort_block: 1 calls 12.263 user 15.834 sys 192.290 real
write_chunk: 1527 calls 11.119 user 0.376 sys 14.344 real
overall: 2568552448 mem 615.947 user 33.593 sys 743.141 real
ls: cannot access '*.cram': No such file or directory