cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o HCC1395_tumor.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1790.347 CPU sec, 66.838 real sec [M::process] read 125750 sequences (18108531 bp) in 0.181 CPU sec, 0.190 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 43991, 1, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 273, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (298.32, 149.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1026) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 125750 reads in 11.848 CPU sec, 0.400 real sec os version: Linux 6.12.83 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 125750 overall: 68488810496 mem 1684.087 user 123.913 sys 73.047 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.HCC1395_tumor.L008\tPU:L008\tSM:HCC1395_tumor\tLB:HCC1395_tumor\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 HCC1395_tumor_R1.fastq.gz HCC1395_tumor_R2.fastq.gz [main] Real time: 73.081 sec; CPU: 1808.003 sec sorting block 0, #reads = 125912 mem = 60232803 peak = 166821888 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.83 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 7336702 output reads: 125912 bam_mem_sort: 1 calls 0.025 user 0.000 sys 0.026 real bam_write: 1 calls 0.089 user 0.009 sys 0.099 real execute: 1 calls 0.058 user 0.113 sys 73.470 real merge_files: 1 calls 0.042 user 0.091 sys 0.254 real parse_chunk: 2 calls 0.057 user 0.010 sys 0.073 real read_chunk: 8 calls 0.044 user 0.019 sys 5.738 real sort_block: 1 calls 0.179 user 0.020 sys 5.774 real write_chunk: 4 calls 0.012 user 0.003 sys 0.034 real overall: 484614144 mem 1.250 user 0.268 sys 73.494 real ls: cannot access '*.cram': No such file or directory