cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o Sig_18_tissue.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1592.779 CPU sec, 56.996 real sec [M::process] read 124610 sequences (17629509 bp) in 0.168 CPU sec, 0.170 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 46577, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 224, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (246.13, 119.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 124610 reads in 18.183 CPU sec, 0.598 real sec os version: Linux 6.12.83 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 124610 overall: 68504641536 mem 1475.902 user 141.949 sys 64.489 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.Sig_18_tissue.L005\tPU:L005\tSM:Sig_18_tissue\tLB:Sig_18_tissue\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 Sig_18_tissue_R1.fastq.gz Sig_18_tissue_R2.fastq.gz [main] Real time: 64.526 sec; CPU: 1617.853 sec sorting block 0, #reads = 124855 mem = 59334865 peak = 166838272 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.83 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 7013233 output reads: 124855 bam_mem_sort: 1 calls 0.027 user 0.000 sys 0.028 real bam_write: 1 calls 0.068 user 0.001 sys 0.072 real execute: 1 calls 0.075 user 0.117 sys 64.905 real merge_files: 1 calls 0.051 user 0.093 sys 0.264 real parse_chunk: 2 calls 0.036 user 0.028 sys 0.071 real read_chunk: 7 calls 0.033 user 0.034 sys 6.848 real sort_block: 1 calls 0.113 user 0.057 sys 6.856 real write_chunk: 3 calls 0.020 user 0.007 sys 0.033 real overall: 471363584 mem 1.231 user 0.313 sys 64.925 real ls: cannot access '*.cram': No such file or directory