This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o HCC1395_BL.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1786.259 CPU sec, 62.050 real sec [M::process] read 124904 sequences (18031367 bp) in 0.168 CPU sec, 0.174 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 44581, 0, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 282, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840) [M::mem_pestat] mean and std.dev: (307.00, 153.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 124904 reads in 20.967 CPU sec, 0.691 real sec os version: Linux 6.12.83 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 124904 overall: 68517191680 mem 1656.917 user 155.656 sys 68.098 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.HCC1395_BL.L007\tPU:L007\tSM:HCC1395_BL\tLB:HCC1395_BL\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 HCC1395_BL_R1.fastq.gz HCC1395_BL_R2.fastq.gz [main] Real time: 68.131 sec; CPU: 1812.575 sec sorting block 0, #reads = 125063 mem = 59373495 peak = 168394752 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.83 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 7316072 output reads: 125063 bam_mem_sort: 1 calls 0.024 user 0.000 sys 0.029 real bam_write: 1 calls 0.084 user 0.009 sys 0.088 real execute: 1 calls 0.053 user 0.121 sys 68.506 real merge_files: 1 calls 0.032 user 0.102 sys 0.249 real parse_chunk: 2 calls 0.055 user 0.000 sys 0.071 real read_chunk: 7 calls 0.029 user 0.033 sys 5.301 real sort_block: 1 calls 0.159 user 0.030 sys 5.332 real write_chunk: 3 calls 0.000 user 0.023 sys 0.030 real overall: 475037696 mem 1.235 user 0.300 sys 68.529 real ls: cannot access '*.cram': No such file or directory