This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o HCC1395_tumor.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1509.136 CPU sec, 51.632 real sec [M::process] read 125626 sequences (18098646 bp) in 0.193 CPU sec, 0.202 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 44052, 2, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 274, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (299.15, 150.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 125626 reads in 13.423 CPU sec, 0.444 real sec os version: Linux 6.12.83 cpu vendor: GenuineIntel signature: 000606a6 features: 1f8bfbff fffa3203 extended: f1bf07ab 00407f5a amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) Platinum 8375C CPU @ 2.90GHz threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 125626 overall: 68566081536 mem 1478.139 user 49.994 sys 57.666 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.HCC1395_tumor.L002\tPU:L002\tSM:HCC1395_tumor\tLB:HCC1395_tumor\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 HCC1395_tumor_R1.fastq.gz HCC1395_tumor_R2.fastq.gz [main] Real time: 57.699 sec; CPU: 1528.136 sec sorting block 0, #reads = 125801 mem = 60183308 peak = 170864640 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.83 cpu vendor: GenuineIntel signature: 000606a6 features: 1f8bfbff fffa3203 extended: f1bf07ab 00407f5a amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) Platinum 8375C CPU @ 2.90GHz threads: 31 max 32 algo: util-sort license: sentieon:util=1 output file size: 7344186 output reads: 125801 bam_mem_sort: 1 calls 0.036 user 0.000 sys 0.040 real bam_write: 1 calls 0.027 user 0.000 sys 0.023 real execute: 1 calls 0.065 user 0.125 sys 58.063 real merge_files: 1 calls 0.060 user 0.090 sys 0.284 real parse_chunk: 2 calls 0.078 user 0.000 sys 0.085 real read_chunk: 8 calls 0.029 user 0.034 sys 5.527 real sort_block: 1 calls 0.124 user 0.024 sys 5.489 real write_chunk: 4 calls 0.013 user 0.008 sys 0.036 real overall: 509485056 mem 1.749 user 0.245 sys 58.081 real ls: cannot access '*.cram': No such file or directory