This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o Sig_18_Blood.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1645.547 CPU sec, 57.286 real sec [M::process] read 124736 sequences (18072176 bp) in 0.172 CPU sec, 0.175 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44863, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 289, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (315.26, 157.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1101) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 124736 reads in 19.936 CPU sec, 0.664 real sec os version: Linux 6.12.83 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 124736 overall: 68495204352 mem 1546.557 user 124.122 sys 63.150 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.Sig_18_Blood.L007\tPU:L007\tSM:Sig_18_Blood\tLB:Sig_18_Blood\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 Sig_18_Blood_R1.fastq.gz Sig_18_Blood_R2.fastq.gz [main] Real time: 63.186 sec; CPU: 1670.682 sec sorting block 0, #reads = 124877 mem = 60037142 peak = 166875136 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.83 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 7455819 output reads: 124877 bam_mem_sort: 1 calls 0.021 user 0.000 sys 0.027 real bam_write: 1 calls 0.033 user 0.000 sys 0.029 real execute: 1 calls 0.065 user 0.119 sys 63.512 real merge_files: 1 calls 0.045 user 0.100 sys 0.263 real parse_chunk: 2 calls 0.023 user 0.032 sys 0.071 real read_chunk: 8 calls 0.047 user 0.027 sys 5.148 real sort_block: 1 calls 0.092 user 0.047 sys 5.110 real write_chunk: 4 calls 0.016 user 0.015 sys 0.036 real overall: 482353152 mem 1.345 user 0.267 sys 63.534 real ls: cannot access '*.cram': No such file or directory