cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o HCC1395_tumor.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1823.905 CPU sec, 63.951 real sec [M::process] read 125124 sequences (17999868 bp) in 0.171 CPU sec, 0.172 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 43763, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 272, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (296.95, 149.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 125124 reads in 23.142 CPU sec, 0.766 real sec os version: Linux 6.12.83 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 125124 overall: 68501516288 mem 1680.870 user 172.948 sys 71.500 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.HCC1395_tumor.L007\tPU:L007\tSM:HCC1395_tumor\tLB:HCC1395_tumor\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 HCC1395_tumor_R1.fastq.gz HCC1395_tumor_R2.fastq.gz [main] Real time: 71.535 sec; CPU: 1853.820 sec sorting block 0, #reads = 125293 mem = 59924104 peak = 168423424 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.83 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 7303558 output reads: 125293 bam_mem_sort: 1 calls 0.023 user 0.000 sys 0.026 real bam_write: 1 calls 0.093 user 0.000 sys 0.093 real execute: 1 calls 0.056 user 0.121 sys 71.922 real merge_files: 1 calls 0.039 user 0.098 sys 0.257 real parse_chunk: 2 calls 0.066 user 0.000 sys 0.073 real read_chunk: 8 calls 0.032 user 0.056 sys 6.729 real sort_block: 1 calls 0.159 user 0.042 sys 6.762 real write_chunk: 4 calls 0.015 user 0.009 sys 0.035 real overall: 484323328 mem 1.261 user 0.250 sys 71.944 real ls: cannot access '*.cram': No such file or directory