This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o Sig_18_tissue.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1356.893 CPU sec, 49.318 real sec [M::process] read 124084 sequences (17558398 bp) in 0.192 CPU sec, 0.195 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 46664, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 224, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (246.33, 120.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 124084 reads in 11.772 CPU sec, 0.408 real sec os version: Linux 6.12.83 cpu vendor: GenuineIntel signature: 000606a6 features: 1f8bfbff fffa320b extended: f1bf07ab 00407f5a amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) Platinum 8375C CPU @ 2.90GHz threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 124084 overall: 68538171392 mem 1257.419 user 117.900 sys 56.390 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.Sig_18_tissue.L008\tPU:L008\tSM:Sig_18_tissue\tLB:Sig_18_tissue\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 Sig_18_tissue_R1.fastq.gz Sig_18_tissue_R2.fastq.gz [main] Real time: 56.422 sec; CPU: 1375.322 sec sorting block 0, #reads = 124306 mem = 59072915 peak = 167002112 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.83 cpu vendor: GenuineIntel signature: 000606a6 features: 1f8bfbff fffa320b extended: f1bf07ab 00407f5a amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) Platinum 8375C CPU @ 2.90GHz threads: 31 max 128 algo: util-sort license: sentieon:util=1 output file size: 6990035 output reads: 124306 bam_mem_sort: 1 calls 0.028 user 0.000 sys 0.030 real bam_write: 1 calls 0.107 user 0.002 sys 0.117 real execute: 1 calls 0.070 user 0.133 sys 56.872 real merge_files: 1 calls 0.051 user 0.105 sys 0.281 real parse_chunk: 2 calls 0.043 user 0.034 sys 0.083 real read_chunk: 7 calls 0.024 user 0.042 sys 6.601 real sort_block: 1 calls 0.163 user 0.067 sys 6.654 real write_chunk: 3 calls 0.034 user 0.000 sys 0.055 real overall: 483745792 mem 1.371 user 0.309 sys 56.888 real ls: cannot access '*.cram': No such file or directory