This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o HCC1395_tumor.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1403.289 CPU sec, 77.638 real sec [M::process] read 125750 sequences (18108531 bp) in 0.201 CPU sec, 0.201 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 43991, 1, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 273, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (298.32, 149.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1026) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 125750 reads in 16.721 CPU sec, 0.560 real sec os version: Linux 6.12.83 cpu vendor: GenuineIntel signature: 000606a6 features: 1f8bfbff fffa320b extended: f1bf07ab 00407f5a amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) Platinum 8375C CPU @ 2.90GHz threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 125750 overall: 68527706112 mem 1303.098 user 124.262 sys 85.553 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.HCC1395_tumor.L008\tPU:L008\tSM:HCC1395_tumor\tLB:HCC1395_tumor\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 HCC1395_tumor_R1.fastq.gz HCC1395_tumor_R2.fastq.gz [main] Real time: 85.587 sec; CPU: 1427.364 sec sorting block 0, #reads = 125912 mem = 60232803 peak = 169050112 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.83 cpu vendor: GenuineIntel signature: 000606a6 features: 1f8bfbff fffa320b extended: f1bf07ab 00407f5a amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) Platinum 8375C CPU @ 2.90GHz threads: 31 max 128 algo: util-sort license: sentieon:util=1 output file size: 7336702 output reads: 125912 bam_mem_sort: 1 calls 0.024 user 0.000 sys 0.030 real bam_write: 1 calls 0.129 user 0.001 sys 0.132 real execute: 1 calls 0.104 user 0.091 sys 86.049 real merge_files: 1 calls 0.089 user 0.066 sys 0.284 real parse_chunk: 2 calls 0.071 user 0.006 sys 0.086 real read_chunk: 8 calls 0.023 user 0.052 sys 7.288 real sort_block: 1 calls 0.191 user 0.057 sys 7.352 real write_chunk: 4 calls 0.023 user 0.005 sys 0.062 real overall: 492961792 mem 1.446 user 0.287 sys 86.071 real ls: cannot access '*.cram': No such file or directory