cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o Sig_18_tissue.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1618.492 CPU sec, 57.743 real sec [M::process] read 124610 sequences (17629509 bp) in 0.164 CPU sec, 0.172 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 46577, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 224, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (246.13, 119.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 124610 reads in 10.022 CPU sec, 0.334 real sec os version: Linux 6.12.83 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 124610 overall: 68520378368 mem 1506.619 user 127.101 sys 63.292 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.Sig_18_tissue.L005\tPU:L005\tSM:Sig_18_tissue\tLB:Sig_18_tissue\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 Sig_18_tissue_R1.fastq.gz Sig_18_tissue_R2.fastq.gz [main] Real time: 63.328 sec; CPU: 1633.723 sec sorting block 0, #reads = 124855 mem = 59334865 peak = 166850560 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.83 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 7013233 output reads: 124855 bam_mem_sort: 1 calls 0.021 user 0.000 sys 0.025 real bam_write: 1 calls 0.056 user 0.006 sys 0.060 real execute: 1 calls 0.054 user 0.125 sys 63.673 real merge_files: 1 calls 0.043 user 0.094 sys 0.250 real parse_chunk: 2 calls 0.046 user 0.011 sys 0.068 real read_chunk: 7 calls 0.029 user 0.044 sys 5.158 real sort_block: 1 calls 0.097 user 0.058 sys 5.157 real write_chunk: 3 calls 0.000 user 0.023 sys 0.031 real overall: 474308608 mem 1.163 user 0.350 sys 63.692 real ls: cannot access '*.cram': No such file or directory