cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o HCC1395_tumor.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1855.161 CPU sec, 64.789 real sec [M::process] read 124464 sequences (17920568 bp) in 0.166 CPU sec, 0.174 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 43611, 0, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 272, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (297.21, 149.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 124464 reads in 24.109 CPU sec, 0.819 real sec os version: Linux 6.12.83 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 124464 overall: 68473155584 mem 1717.002 user 168.501 sys 71.845 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.HCC1395_tumor.L003\tPU:L003\tSM:HCC1395_tumor\tLB:HCC1395_tumor\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 HCC1395_tumor_R1.fastq.gz HCC1395_tumor_R2.fastq.gz [main] Real time: 71.903 sec; CPU: 1885.506 sec sorting block 0, #reads = 124643 mem = 59577413 peak = 165244928 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.83 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 7263380 output reads: 124643 bam_mem_sort: 1 calls 0.031 user 0.000 sys 0.034 real bam_write: 1 calls 0.044 user 0.000 sys 0.042 real execute: 1 calls 0.074 user 0.103 sys 72.248 real merge_files: 1 calls 0.058 user 0.079 sys 0.257 real parse_chunk: 2 calls 0.065 user 0.025 sys 0.101 real read_chunk: 7 calls 0.023 user 0.064 sys 6.182 real sort_block: 1 calls 0.097 user 0.076 sys 6.183 real write_chunk: 3 calls 0.010 user 0.018 sys 0.034 real overall: 467693568 mem 1.319 user 0.273 sys 72.282 real ls: cannot access '*.cram': No such file or directory