This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o Sig_18_Blood.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 2607.956 CPU sec, 97.982 real sec [M::process] read 125148 sequences (18140009 bp) in 0.175 CPU sec, 0.183 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 45084, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 290, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (315.67, 157.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1091) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 125148 reads in 31.060 CPU sec, 1.013 real sec os version: Linux 6.12.83 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 125148 overall: 68045295616 mem 2174.383 user 472.541 sys 106.931 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.Sig_18_Blood.L006\tPU:L006\tSM:Sig_18_Blood\tLB:Sig_18_Blood\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 Sig_18_Blood_R1.fastq.gz Sig_18_Blood_R2.fastq.gz [main] Real time: 106.960 sec; CPU: 2646.927 sec sorting block 0, #reads = 125303 mem = 60297184 peak = 160493568 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.83 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 384 algo: util-sort license: sentieon:util=1 output file size: 7517831 output reads: 125303 bam_mem_sort: 1 calls 0.033 user 0.000 sys 0.035 real bam_write: 1 calls 0.048 user 0.000 sys 0.046 real execute: 1 calls 0.079 user 0.134 sys 107.373 real merge_files: 1 calls 0.055 user 0.108 sys 0.296 real parse_chunk: 2 calls 0.080 user 0.000 sys 0.094 real read_chunk: 8 calls 0.027 user 0.067 sys 7.884 real sort_block: 1 calls 0.142 user 0.052 sys 7.880 real write_chunk: 4 calls 0.018 user 0.011 sys 0.038 real overall: 456560640 mem 1.471 user 0.301 sys 107.388 real ls: cannot access '*.cram': No such file or directory