cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o Sig_18_Blood.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2735.968 CPU sec, 109.306 real sec
[M::process] read 125180 sequences (18146427 bp) in 0.176 CPU sec, 0.181 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44859, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 289, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863)
[M::mem_pestat] mean and std.dev: (315.14, 156.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1087)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 125180 reads in 26.313 CPU sec, 0.862 real sec
os version: Linux 6.12.83
cpu vendor: GenuineIntel
signature: 000a06d1
features: 1f8bfbff fffab20b
extended: f1bf27eb 1b407f7e
amd bits: 2c100800 00000121
brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 125180
overall: 67979239424 mem 2215.237 user 554.307 sys 117.414 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.Sig_18_Blood.L003\tPU:L003\tSM:Sig_18_Blood\tLB:Sig_18_Blood\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 Sig_18_Blood_R1.fastq.gz Sig_18_Blood_R2.fastq.gz
[main] Real time: 117.500 sec; CPU: 2769.547 sec
sorting block 0, #reads = 125327 mem = 60250287 peak = 163651584
uniq header count 1
merging 1 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.83
cpu vendor: GenuineIntel
signature: 000a06d1
features: 1f8bfbff fffab20b
extended: f1bf27eb 1b407f7e
amd bits: 2c100800 00000121
brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 7480298
output reads: 125327
bam_mem_sort: 1 calls 0.028 user 0.001 sys 0.033 real
bam_write: 1 calls 0.074 user 0.014 sys 0.093 real
execute: 1 calls 0.053 user 0.150 sys 117.864 real
merge_files: 1 calls 0.031 user 0.128 sys 0.278 real
parse_chunk: 2 calls 0.050 user 0.022 sys 0.079 real
read_chunk: 8 calls 0.051 user 0.058 sys 7.186 real
sort_block: 1 calls 0.130 user 0.080 sys 7.221 real
write_chunk: 4 calls 0.012 user 0.022 sys 0.039 real
overall: 464736256 mem 1.292 user 0.400 sys 117.944 real
ls: cannot access '*.cram': No such file or directory