This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o Sig_18_tissue.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 2486.822 CPU sec, 100.516 real sec [M::process] read 124960 sequences (17676029 bp) in 0.166 CPU sec, 0.176 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 46672, 3, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 223, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (246.09, 119.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 124960 reads in 29.890 CPU sec, 0.980 real sec os version: Linux 6.12.83 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 124960 overall: 67982336000 mem 2090.914 user 439.159 sys 114.868 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.Sig_18_tissue.L006\tPU:L006\tSM:Sig_18_tissue\tLB:Sig_18_tissue\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 Sig_18_tissue_R1.fastq.gz Sig_18_tissue_R2.fastq.gz [main] Real time: 114.906 sec; CPU: 2530.080 sec sorting block 0, #reads = 125203 mem = 59559176 peak = 160546816 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.83 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 384 algo: util-sort license: sentieon:util=1 output file size: 7072948 output reads: 125203 bam_mem_sort: 1 calls 0.028 user 0.001 sys 0.037 real bam_write: 1 calls 0.081 user 0.014 sys 0.089 real execute: 1 calls 0.054 user 0.148 sys 115.329 real merge_files: 1 calls 0.034 user 0.121 sys 0.274 real parse_chunk: 2 calls 0.043 user 0.037 sys 0.081 real read_chunk: 8 calls 0.027 user 0.061 sys 13.318 real sort_block: 1 calls 0.135 user 0.082 sys 13.367 real write_chunk: 4 calls 0.011 user 0.020 sys 0.040 real overall: 467722240 mem 1.183 user 0.400 sys 115.351 real ls: cannot access '*.cram': No such file or directory