cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o Sig_18_Blood.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1551.789 CPU sec, 54.756 real sec [M::process] read 124416 sequences (18040112 bp) in 0.181 CPU sec, 0.185 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 44650, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 290, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (315.46, 157.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1087) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 124416 reads in 11.825 CPU sec, 0.408 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 124416 overall: 68521947136 mem 1441.270 user 128.234 sys 61.057 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.Sig_18_Blood.L002\tPU:L002\tSM:Sig_18_Blood\tLB:Sig_18_Blood\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 Sig_18_Blood_R1.fastq.gz Sig_18_Blood_R2.fastq.gz [main] Real time: 61.112 sec; CPU: 1569.507 sec sorting block 0, #reads = 124553 mem = 60004382 peak = 168439808 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 7487684 output reads: 124553 bam_mem_sort: 1 calls 0.021 user 0.000 sys 0.026 real bam_write: 1 calls 0.072 user 0.006 sys 0.076 real execute: 1 calls 0.023 user 0.152 sys 61.450 real merge_files: 1 calls 0.018 user 0.118 sys 0.253 real parse_chunk: 2 calls 0.039 user 0.014 sys 0.071 real read_chunk: 8 calls 0.049 user 0.030 sys 5.828 real sort_block: 1 calls 0.124 user 0.048 sys 5.842 real write_chunk: 4 calls 0.009 user 0.013 sys 0.031 real overall: 476241920 mem 1.282 user 0.250 sys 61.497 real ls: cannot access '*.cram': No such file or directory