cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o HCC1395_BL.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1593.353 CPU sec, 56.145 real sec [M::process] read 124700 sequences (17992902 bp) in 0.172 CPU sec, 0.180 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 44507, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 282, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849) [M::mem_pestat] mean and std.dev: (307.31, 153.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1070) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 124700 reads in 11.572 CPU sec, 0.400 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 124700 overall: 68523466752 mem 1477.007 user 133.758 sys 62.385 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.HCC1395_BL.L002\tPU:L002\tSM:HCC1395_BL\tLB:HCC1395_BL\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 HCC1395_BL_R1.fastq.gz HCC1395_BL_R2.fastq.gz [main] Real time: 62.458 sec; CPU: 1610.768 sec sorting block 0, #reads = 124816 mem = 59282090 peak = 168386560 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 7313733 output reads: 124816 bam_mem_sort: 1 calls 0.025 user 0.000 sys 0.027 real bam_write: 1 calls 0.091 user 0.002 sys 0.097 real execute: 1 calls 0.088 user 0.080 sys 62.807 real merge_files: 1 calls 0.065 user 0.063 sys 0.242 real parse_chunk: 2 calls 0.037 user 0.027 sys 0.069 real read_chunk: 7 calls 0.050 user 0.036 sys 5.774 real sort_block: 1 calls 0.144 user 0.054 sys 5.814 real write_chunk: 3 calls 0.012 user 0.004 sys 0.029 real overall: 471912448 mem 1.267 user 0.229 sys 62.857 real ls: cannot access '*.cram': No such file or directory