This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o HCC1395_BL.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 2833.472 CPU sec, 114.313 real sec [M::process] read 124828 sequences (18022863 bp) in 0.182 CPU sec, 0.189 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44466, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 282, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837) [M::mem_pestat] mean and std.dev: (306.65, 153.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 124828 reads in 37.148 CPU sec, 1.219 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 124828 overall: 68133355520 mem 2260.336 user 616.977 sys 122.230 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.HCC1395_BL.L006\tPU:L006\tSM:HCC1395_BL\tLB:HCC1395_BL\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 HCC1395_BL_R1.fastq.gz HCC1395_BL_R2.fastq.gz [main] Real time: 122.262 sec; CPU: 2877.316 sec sorting block 0, #reads = 124937 mem = 59360983 peak = 160550912 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 384 algo: util-sort license: sentieon:util=1 output file size: 7327533 output reads: 124937 bam_mem_sort: 1 calls 0.040 user 0.000 sys 0.047 real bam_write: 1 calls 0.042 user 0.009 sys 0.044 real execute: 1 calls 0.024 user 0.214 sys 122.708 real merge_files: 1 calls 0.024 user 0.164 sys 0.321 real parse_chunk: 2 calls 0.095 user 0.009 sys 0.121 real read_chunk: 7 calls 0.025 user 0.056 sys 6.663 real sort_block: 1 calls 0.135 user 0.063 sys 6.652 real write_chunk: 3 calls 0.000 user 0.052 sys 0.057 real overall: 423276544 mem 1.608 user 0.502 sys 122.727 real ls: cannot access '*.cram': No such file or directory