This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o HCC1395_tumor.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1802.401 CPU sec, 63.892 real sec [M::process] read 124926 sequences (17971193 bp) in 0.171 CPU sec, 0.177 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 43938, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 272, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (297.73, 149.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 124926 reads in 19.680 CPU sec, 0.655 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 124926 overall: 68479418368 mem 1705.792 user 121.401 sys 69.667 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.HCC1395_tumor.L005\tPU:L005\tSM:HCC1395_tumor\tLB:HCC1395_tumor\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 HCC1395_tumor_R1.fastq.gz HCC1395_tumor_R2.fastq.gz [main] Real time: 69.702 sec; CPU: 1827.196 sec sorting block 0, #reads = 125074 mem = 59766436 peak = 168386560 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 7280382 output reads: 125074 bam_mem_sort: 1 calls 0.019 user 0.000 sys 0.026 real bam_write: 1 calls 0.031 user 0.000 sys 0.025 real execute: 1 calls 0.057 user 0.133 sys 70.034 real merge_files: 1 calls 0.045 user 0.100 sys 0.268 real parse_chunk: 2 calls 0.063 user 0.009 sys 0.078 real read_chunk: 8 calls 0.007 user 0.049 sys 5.069 real sort_block: 1 calls 0.071 user 0.052 sys 5.023 real write_chunk: 4 calls 0.016 user 0.017 sys 0.037 real overall: 480485376 mem 1.417 user 0.317 sys 70.053 real ls: cannot access '*.cram': No such file or directory