cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o HCC1395_BL.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1766.690 CPU sec, 62.273 real sec [M::process] read 125588 sequences (18133711 bp) in 0.179 CPU sec, 0.182 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 44648, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 284, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845) [M::mem_pestat] mean and std.dev: (308.41, 153.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1064) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 125588 reads in 26.364 CPU sec, 0.863 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 125588 overall: 68469997568 mem 1634.534 user 165.665 sys 70.304 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.HCC1395_BL.L005\tPU:L005\tSM:HCC1395_BL\tLB:HCC1395_BL\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 HCC1395_BL_R1.fastq.gz HCC1395_BL_R2.fastq.gz [main] Real time: 70.338 sec; CPU: 1800.201 sec sorting block 0, #reads = 125732 mem = 59740910 peak = 165216256 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 7392989 output reads: 125732 bam_mem_sort: 1 calls 0.031 user 0.000 sys 0.034 real bam_write: 1 calls 0.018 user 0.008 sys 0.024 real execute: 1 calls 0.105 user 0.106 sys 70.706 real merge_files: 1 calls 0.075 user 0.089 sys 0.290 real parse_chunk: 2 calls 0.054 user 0.043 sys 0.105 real read_chunk: 7 calls 0.023 user 0.047 sys 7.112 real sort_block: 1 calls 0.079 user 0.060 sys 7.074 real write_chunk: 3 calls 0.009 user 0.015 sys 0.035 real overall: 474333184 mem 1.569 user 0.304 sys 70.726 real ls: cannot access '*.cram': No such file or directory