This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o HCC1395_tumor.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1749.676 CPU sec, 70.461 real sec [M::process] read 125052 sequences (17995734 bp) in 0.191 CPU sec, 0.201 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 43482, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 272, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (297.47, 149.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 125052 reads in 25.352 CPU sec, 0.837 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000606a6 features: 1f8bfbff fffa320b extended: f1bf07ab 00407f5a amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) Platinum 8375C CPU @ 2.90GHz threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 125052 overall: 68505726976 mem 1505.360 user 277.414 sys 79.059 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.HCC1395_tumor.L006\tPU:L006\tSM:HCC1395_tumor\tLB:HCC1395_tumor\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 HCC1395_tumor_R1.fastq.gz HCC1395_tumor_R2.fastq.gz [main] Real time: 79.097 sec; CPU: 1782.781 sec sorting block 0, #reads = 125216 mem = 59825649 peak = 166977536 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000606a6 features: 1f8bfbff fffa320b extended: f1bf07ab 00407f5a amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) Platinum 8375C CPU @ 2.90GHz threads: 31 max 128 algo: util-sort license: sentieon:util=1 output file size: 7277935 output reads: 125216 bam_mem_sort: 1 calls 0.054 user 0.000 sys 0.055 real bam_write: 1 calls 0.039 user 0.002 sys 0.044 real execute: 1 calls 0.080 user 0.154 sys 79.594 real merge_files: 1 calls 0.070 user 0.122 sys 0.344 real parse_chunk: 2 calls 0.073 user 0.043 sys 0.124 real read_chunk: 8 calls 0.010 user 0.075 sys 7.727 real sort_block: 1 calls 0.154 user 0.077 sys 7.735 real write_chunk: 4 calls 0.016 user 0.008 sys 0.034 real overall: 488591360 mem 1.727 user 0.414 sys 79.618 real ls: cannot access '*.cram': No such file or directory