cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o Sig_18_tissue.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1363.002 CPU sec, 46.895 real sec [M::process] read 125154 sequences (17709424 bp) in 0.204 CPU sec, 0.206 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 46750, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 224, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (245.57, 119.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 125154 reads in 11.090 CPU sec, 0.371 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000c06f2 features: 1f8bfbff fffab20b extended: f1bf07ab 1a407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) Platinum 8559C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 125154 overall: 68558823424 mem 1329.003 user 49.921 sys 52.114 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.Sig_18_tissue.L001\tPU:L001\tSM:Sig_18_tissue\tLB:Sig_18_tissue\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 Sig_18_tissue_R1.fastq.gz Sig_18_tissue_R2.fastq.gz [main] Real time: 52.159 sec; CPU: 1378.926 sec sorting block 0, #reads = 125386 mem = 59501897 peak = 169820160 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000c06f2 features: 1f8bfbff fffab20b extended: f1bf07ab 1a407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) Platinum 8559C threads: 31 max 64 algo: util-sort license: sentieon:util=1 output file size: 7015338 output reads: 125386 bam_mem_sort: 1 calls 0.023 user 0.000 sys 0.026 real bam_write: 1 calls 0.021 user 0.000 sys 0.018 real execute: 1 calls 0.036 user 0.140 sys 52.448 real merge_files: 1 calls 0.016 user 0.115 sys 0.254 real parse_chunk: 2 calls 0.046 user 0.018 sys 0.080 real read_chunk: 7 calls 0.029 user 0.026 sys 4.760 real sort_block: 1 calls 0.082 user 0.038 sys 4.714 real write_chunk: 3 calls 0.000 user 0.015 sys 0.026 real overall: 493711360 mem 1.357 user 0.248 sys 52.488 real ls: cannot access '*.cram': No such file or directory