cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o Sig_18_tissue.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 2774.179 CPU sec, 116.907 real sec [M::process] read 124610 sequences (17629509 bp) in 0.169 CPU sec, 0.173 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 46577, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 224, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (246.13, 119.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 124610 reads in 47.535 CPU sec, 1.551 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 124610 overall: 68152197120 mem 2081.705 user 749.579 sys 128.023 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.Sig_18_tissue.L005\tPU:L005\tSM:Sig_18_tissue\tLB:Sig_18_tissue\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 Sig_18_tissue_R1.fastq.gz Sig_18_tissue_R2.fastq.gz [main] Real time: 128.055 sec; CPU: 2831.287 sec sorting block 0, #reads = 124855 mem = 59334865 peak = 160497664 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 384 algo: util-sort license: sentieon:util=1 output file size: 7013233 output reads: 124855 bam_mem_sort: 1 calls 0.029 user 0.000 sys 0.032 real bam_write: 1 calls 0.083 user 0.000 sys 0.081 real execute: 1 calls 0.047 user 0.148 sys 128.448 real merge_files: 1 calls 0.027 user 0.118 sys 0.260 real parse_chunk: 2 calls 0.032 user 0.036 sys 0.075 real read_chunk: 7 calls 0.010 user 0.082 sys 9.502 real sort_block: 1 calls 0.138 user 0.074 sys 9.537 real write_chunk: 3 calls 0.008 user 0.014 sys 0.035 real overall: 455626752 mem 1.035 user 0.488 sys 128.466 real ls: cannot access '*.cram': No such file or directory