cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o Sig_18_tissue.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 2275.383 CPU sec, 84.868 real sec [M::process] read 125244 sequences (17742351 bp) in 0.171 CPU sec, 0.173 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 46956, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 223, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (246.06, 119.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 125244 reads in 28.035 CPU sec, 0.930 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 125244 overall: 68274921472 mem 1912.828 user 397.178 sys 92.395 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.Sig_18_tissue.L002\tPU:L002\tSM:Sig_18_tissue\tLB:Sig_18_tissue\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 Sig_18_tissue_R1.fastq.gz Sig_18_tissue_R2.fastq.gz [main] Real time: 92.433 sec; CPU: 2310.009 sec sorting block 0, #reads = 125469 mem = 59623350 peak = 157360128 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 384 algo: util-sort license: sentieon:util=1 output file size: 7030812 output reads: 125469 bam_mem_sort: 1 calls 0.047 user 0.001 sys 0.048 real bam_write: 1 calls 0.059 user 0.010 sys 0.070 real execute: 1 calls 0.076 user 0.173 sys 92.901 real merge_files: 1 calls 0.065 user 0.131 sys 0.317 real parse_chunk: 2 calls 0.081 user 0.027 sys 0.115 real read_chunk: 8 calls 0.050 user 0.055 sys 6.541 real sort_block: 1 calls 0.180 user 0.047 sys 6.578 real write_chunk: 4 calls 0.012 user 0.024 sys 0.040 real overall: 447549440 mem 1.369 user 0.460 sys 92.924 real ls: cannot access '*.cram': No such file or directory