Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/14/25305dacc77e636c8bfb85fd5d6022/output/Sig_18_tissue_S3_L006_R1_001.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/9e/67b2dc2b6eba7c4b53dcd815b48a37/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/14/25305dacc77e636c8bfb85fd5d6022/output/Sig_18_tissue_S3_L006_R2_001.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/9e/67b2dc2b6eba7c4b53dcd815b48a37/.command.run
==> STAGING COMPLETE (4 inputs)

Detecting adapter sequence for read1...
>Illumina TruSeq Adapter Read 1
AGATCGGAAGAGCACACGTCTGAACTCCAGTCA

Detecting adapter sequence for read2...
>Illumina TruSeq Adapter Read 2
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT

Read1 before filtering:
total reads: 62481
total bases: 9372150
Q20 bases: 9210071(98.2706%)
Q30 bases: 8935030(95.336%)
Q40 bases: 8935030(95.336%)

Read2 before filtering:
total reads: 62481
total bases: 9372150
Q20 bases: 9155673(97.6902%)
Q30 bases: 8869801(94.64%)
Q40 bases: 8869801(94.64%)

Read1 after filtering:
total reads: 62480
total bases: 8837869
Q20 bases: 8734680(98.8324%)
Q30 bases: 8498812(96.1636%)
Q40 bases: 8498812(96.1636%)

Read2 after filtering:
total reads: 62480
total bases: 8838160
Q20 bases: 8678041(98.1883%)
Q30 bases: 8445227(95.5541%)
Q40 bases: 8445227(95.5541%)

Filtering result:
reads passed filter: 124960
reads failed due to low quality: 2
reads failed due to too many N: 0
reads failed due to too short: 0
reads with adapter trimmed: 29347
bases trimmed due to adapters: 1068019

Duplication rate: 0.693011%

Insert size peak (evaluated by paired-end reads): 224

JSON report: Sig_18_tissue.fastp.json
HTML report: Sig_18_tissue.fastp.html

fastp --in1 Sig_18_tissue_S3_L006_R1_001.fastq.gz --in2 Sig_18_tissue_S3_L006_R2_001.fastq.gz --out1 Sig_18_tissue/Sig_18_tissue_R1.fastq.gz --out2 Sig_18_tissue/Sig_18_tissue_R2.fastq.gz --detect_adapter_for_pe --length_required 15 --json Sig_18_tissue.fastp.json --html Sig_18_tissue.fastp.html --thread 4 
fastp v1.0.1, time used: 1 seconds