This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o Sig_18_Blood.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1156.813 CPU sec, 40.345 real sec [M::process] read 125180 sequences (18146427 bp) in 0.182 CPU sec, 0.190 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44859, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 289, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (315.14, 156.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1087) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 125180 reads in 9.751 CPU sec, 0.328 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000c06f2 features: 1f8bfbff fffab20b extended: f1bf07ab 1a407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) Platinum 8559C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 125180 overall: 68557209600 mem 1123.460 user 47.933 sys 45.514 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.Sig_18_Blood.L003\tPU:L003\tSM:Sig_18_Blood\tLB:Sig_18_Blood\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 Sig_18_Blood_R1.fastq.gz Sig_18_Blood_R2.fastq.gz [main] Real time: 45.544 sec; CPU: 1171.396 sec sorting block 0, #reads = 125327 mem = 60250287 peak = 169713664 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000c06f2 features: 1f8bfbff fffab20b extended: f1bf07ab 1a407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) Platinum 8559C threads: 31 max 64 algo: util-sort license: sentieon:util=1 output file size: 7480298 output reads: 125327 bam_mem_sort: 1 calls 0.016 user 0.000 sys 0.025 real bam_write: 1 calls 0.028 user 0.000 sys 0.020 real execute: 1 calls 0.065 user 0.114 sys 45.864 real merge_files: 1 calls 0.048 user 0.091 sys 0.262 real parse_chunk: 2 calls 0.061 user 0.010 sys 0.077 real read_chunk: 8 calls 0.031 user 0.031 sys 4.774 real sort_block: 1 calls 0.087 user 0.034 sys 4.728 real write_chunk: 4 calls 0.007 user 0.009 sys 0.030 real overall: 503697408 mem 1.435 user 0.290 sys 45.887 real ls: cannot access '*.cram': No such file or directory