cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o HCC1395_BL.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 2120.360 CPU sec, 75.597 real sec [M::process] read 125182 sequences (18067457 bp) in 0.169 CPU sec, 0.176 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44339, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 284, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (307.42, 153.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1062) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 125182 reads in 24.077 CPU sec, 0.791 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: bfebfbff 7ffefbff extended: f3bfb7ef fb417ffe amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 125182 overall: 68199424000 mem 1833.873 user 317.299 sys 83.131 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.HCC1395_BL.L004\tPU:L004\tSM:HCC1395_BL\tLB:HCC1395_BL\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 HCC1395_BL_R1.fastq.gz HCC1395_BL_R2.fastq.gz [main] Real time: 83.273 sec; CPU: 2151.175 sec sorting block 0, #reads = 125334 mem = 59594110 peak = 163577856 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: bfebfbff 7ffefbff extended: f3bfb7ef fb417ffe amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 384 algo: util-sort license: sentieon:util=1 output file size: 7367948 output reads: 125334 bam_mem_sort: 1 calls 0.025 user 0.000 sys 0.032 real bam_write: 1 calls 0.092 user 0.002 sys 0.092 real execute: 1 calls 0.068 user 0.125 sys 83.601 real merge_files: 1 calls 0.049 user 0.097 sys 0.272 real parse_chunk: 2 calls 0.065 user 0.000 sys 0.077 real read_chunk: 7 calls 0.021 user 0.037 sys 6.696 real sort_block: 1 calls 0.156 user 0.047 sys 6.726 real write_chunk: 3 calls 0.015 user 0.015 sys 0.043 real overall: 453980160 mem 1.407 user 0.261 sys 83.709 real ls: cannot access '*.cram': No such file or directory