This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o HCC1395_tumor.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1294.908 CPU sec, 45.569 real sec [M::process] read 125124 sequences (17999868 bp) in 0.169 CPU sec, 0.175 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 43763, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 272, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (296.95, 149.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 125124 reads in 8.833 CPU sec, 0.299 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000c06f2 features: bfebfbff 7ffefbff extended: f3bfb7ef fb417ffe amd bits: 2c100800 00000121 brand: INTEL(R) XEON(R) PLATINUM 8559C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 125124 overall: 68514050048 mem 1227.695 user 81.136 sys 50.965 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.HCC1395_tumor.L007\tPU:L007\tSM:HCC1395_tumor\tLB:HCC1395_tumor\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 HCC1395_tumor_R1.fastq.gz HCC1395_tumor_R2.fastq.gz [main] Real time: 51.005 sec; CPU: 1308.833 sec sorting block 0, #reads = 125293 mem = 59924104 peak = 168394752 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000c06f2 features: bfebfbff 7ffefbff extended: f3bfb7ef fb417ffe amd bits: 2c100800 00000121 brand: INTEL(R) XEON(R) PLATINUM 8559C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 7303558 output reads: 125293 bam_mem_sort: 1 calls 0.021 user 0.000 sys 0.028 real bam_write: 1 calls 0.111 user 0.008 sys 0.113 real execute: 1 calls 0.066 user 0.126 sys 51.427 real merge_files: 1 calls 0.049 user 0.102 sys 0.269 real parse_chunk: 2 calls 0.049 user 0.016 sys 0.075 real read_chunk: 8 calls 0.024 user 0.027 sys 5.047 real sort_block: 1 calls 0.185 user 0.029 sys 5.097 real write_chunk: 4 calls 0.026 user 0.009 sys 0.047 real overall: 484601856 mem 1.342 user 0.301 sys 51.449 real ls: cannot access '*.cram': No such file or directory