cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o HCC1395_tumor.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 2323.217 CPU sec, 94.696 real sec [M::process] read 124560 sequences (17940725 bp) in 0.168 CPU sec, 0.174 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 43403, 0, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 272, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (297.87, 148.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1025) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 124560 reads in 26.859 CPU sec, 0.881 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 124560 overall: 68082999296 mem 1887.946 user 468.586 sys 102.040 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.HCC1395_tumor.L004\tPU:L004\tSM:HCC1395_tumor\tLB:HCC1395_tumor\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 HCC1395_tumor_R1.fastq.gz HCC1395_tumor_R2.fastq.gz [main] Real time: 102.136 sec; CPU: 2356.534 sec sorting block 0, #reads = 124723 mem = 59715543 peak = 157368320 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 384 algo: util-sort license: sentieon:util=1 output file size: 7289742 output reads: 124723 bam_mem_sort: 1 calls 0.027 user 0.000 sys 0.028 real bam_write: 1 calls 0.031 user 0.002 sys 0.034 real execute: 1 calls 0.064 user 0.147 sys 102.443 real merge_files: 1 calls 0.036 user 0.129 sys 0.284 real parse_chunk: 2 calls 0.043 user 0.032 sys 0.079 real read_chunk: 8 calls 0.025 user 0.060 sys 6.415 real sort_block: 1 calls 0.095 user 0.057 sys 6.383 real write_chunk: 4 calls 0.008 user 0.033 sys 0.044 real overall: 438362112 mem 1.390 user 0.320 sys 102.518 real ls: cannot access '*.cram': No such file or directory