This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o HCC1395_tumor.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 2081.686 CPU sec, 85.440 real sec [M::process] read 125750 sequences (18108531 bp) in 0.175 CPU sec, 0.176 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 43991, 1, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 273, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (298.32, 149.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1026) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 125750 reads in 23.277 CPU sec, 0.765 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 125750 overall: 67988627456 mem 1713.541 user 401.733 sys 96.521 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.HCC1395_tumor.L008\tPU:L008\tSM:HCC1395_tumor\tLB:HCC1395_tumor\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 HCC1395_tumor_R1.fastq.gz HCC1395_tumor_R2.fastq.gz [main] Real time: 96.553 sec; CPU: 2115.278 sec sorting block 0, #reads = 125912 mem = 60232803 peak = 163651584 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 384 algo: util-sort license: sentieon:util=1 output file size: 7336702 output reads: 125912 bam_mem_sort: 1 calls 0.032 user 0.000 sys 0.040 real bam_write: 1 calls 0.055 user 0.000 sys 0.049 real execute: 1 calls 0.069 user 0.156 sys 96.966 real merge_files: 1 calls 0.058 user 0.124 sys 0.301 real parse_chunk: 2 calls 0.066 user 0.001 sys 0.080 real read_chunk: 8 calls 0.019 user 0.051 sys 10.261 real sort_block: 1 calls 0.128 user 0.046 sys 10.266 real write_chunk: 4 calls 0.013 user 0.027 sys 0.044 real overall: 465063936 mem 1.382 user 0.306 sys 96.985 real ls: cannot access '*.cram': No such file or directory