cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o HCC1395_BL.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1730.924 CPU sec, 59.659 real sec
[M::process] read 124828 sequences (18022863 bp) in 0.211 CPU sec, 0.220 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44466, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 282, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (306.65, 153.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 124828 reads in 11.914 CPU sec, 0.400 real sec
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 124828
overall: 68485754880 mem 1646.774 user 103.962 sys 67.975 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.HCC1395_BL.L006\tPU:L006\tSM:HCC1395_BL\tLB:HCC1395_BL\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 HCC1395_BL_R1.fastq.gz HCC1395_BL_R2.fastq.gz
[main] Real time: 68.015 sec; CPU: 1750.739 sec
sorting block 0, #reads = 124937 mem = 59360983 peak = 165249024
uniq header count 1
merging 1 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 7327533
output reads: 124937
bam_mem_sort: 1 calls 0.030 user 0.000 sys 0.039 real
bam_write: 1 calls 0.044 user 0.000 sys 0.036 real
execute: 1 calls 0.074 user 0.139 sys 68.425 real
merge_files: 1 calls 0.053 user 0.115 sys 0.309 real
parse_chunk: 2 calls 0.083 user 0.027 sys 0.113 real
read_chunk: 7 calls 0.032 user 0.027 sys 7.840 real
sort_block: 1 calls 0.146 user 0.021 sys 7.815 real
write_chunk: 3 calls 0.007 user 0.023 sys 0.038 real
overall: 470896640 mem 1.587 user 0.315 sys 68.450 real
ls: cannot access '*.cram': No such file or directory