This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o NA12878_c_0002_gDNA_0004_B23KGCJLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1516.062 CPU sec, 469.825 real sec
[M::process] read 684682 sequences (100000185 bp) in 0.921 CPU sec, 0.922 real sec...
[M::process] read 684580 sequences (100000027 bp) in 1.037 CPU sec, 1.057 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 284518, 0, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 160, 174)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (87, 232)
[M::mem_pestat] mean and std.dev: (153.67, 18.93)
[M::mem_pestat] low and high boundaries for proper pairs: (58, 261)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 300, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860)
[M::mem_pestat] mean and std.dev: (319.61, 152.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1080)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 684466 sequences (100000294 bp) in 1.353 CPU sec, 1.381 real sec...
[M::process] read 683718 sequences (100000013 bp) in 1.232 CPU sec, 1.247 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285249, 2, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 299, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862)
[M::mem_pestat] mean and std.dev: (319.70, 153.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1083)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (109, 147, 198)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376)
[M::mem_pestat] mean and std.dev: (157.81, 64.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 465)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684682 reads in 131.929 CPU sec, 4.203 real sec
[M::mem_process_seqs] Processed 684580 reads in 81.333 CPU sec, 2.621 real sec
sorting block 0, #reads = 1072442 mem = 536880802 peak = 619065344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284546, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 302, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (322.29, 154.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1093)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 173, 212)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (29, 334)
[M::mem_pestat] mean and std.dev: (188.30, 53.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 403)
[M::mem_pestat] skip orientation RR
[M::process] read 683744 sequences (100000082 bp) in 1.747 CPU sec, 1.811 real sec...
[M::process] read 683538 sequences (100000080 bp) in 1.120 CPU sec, 1.120 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284679, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 304, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875)
[M::mem_pestat] mean and std.dev: (324.82, 155.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1099)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684466 reads in 124.141 CPU sec, 3.949 real sec
[M::mem_process_seqs] Processed 683718 reads in 78.763 CPU sec, 2.522 real sec
sorting block 1, #reads = 1072480 mem = 536877113 peak = 744894464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284566, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 303, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (324.63, 156.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683108 sequences (100000186 bp) in 1.848 CPU sec, 1.917 real sec...
[M::process] read 684046 sequences (100000038 bp) in 1.343 CPU sec, 1.366 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284465, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 300, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867)
[M::mem_pestat] mean and std.dev: (322.38, 155.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1089)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683744 reads in 133.691 CPU sec, 4.260 real sec
[M::mem_process_seqs] Processed 683538 reads in 87.235 CPU sec, 2.781 real sec
sorting block 2, #reads = 1072145 mem = 536880811 peak = 798814208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284041, 1, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 207, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (219.30, 80.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 303, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875)
[M::mem_pestat] mean and std.dev: (325.08, 156.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1099)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 684834 sequences (100000185 bp) in 2.101 CPU sec, 2.195 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284520, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 305, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871)
[M::mem_pestat] mean and std.dev: (323.70, 153.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1094)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684320 sequences (100000300 bp) in 1.680 CPU sec, 1.679 real sec...
[M::mem_process_seqs] Processed 683108 reads in 138.556 CPU sec, 4.403 real sec
sorting block 3, #reads = 1070866 mem = 536880662 peak = 803254272
[M::mem_process_seqs] Processed 684046 reads in 87.411 CPU sec, 2.782 real sec
sorting block 4, #reads = 1071538 mem = 536882716 peak = 845701120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284357, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 302, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (321.82, 154.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1103)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 286, 715)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1773)
[M::mem_pestat] mean and std.dev: (391.17, 302.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2302)
[M::mem_pestat] skip orientation RR
[M::process] read 683812 sequences (100000098 bp) in 1.750 CPU sec, 1.866 real sec...
[M::process] read 683604 sequences (100000280 bp) in 1.240 CPU sec, 1.248 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284300, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 304, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879)
[M::mem_pestat] mean and std.dev: (323.85, 155.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1105)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684834 reads in 139.160 CPU sec, 4.448 real sec
[M::mem_process_seqs] Processed 684320 reads in 88.405 CPU sec, 2.820 real sec
sorting block 5, #reads = 1072624 mem = 536878198 peak = 845701120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284026, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 306, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (325.88, 156.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683910 sequences (100000201 bp) in 2.484 CPU sec, 2.645 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284429, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 305, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881)
[M::mem_pestat] mean and std.dev: (325.85, 156.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1107)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683812 reads in 136.749 CPU sec, 4.361 real sec
[M::mem_process_seqs] Processed 683604 reads in 88.337 CPU sec, 2.790 real sec
[M::process] read 683490 sequences (100000097 bp) in 2.042 CPU sec, 2.115 real sec...
sorting block 6, #reads = 1071827 mem = 536882425 peak = 845701120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284548, 1, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 301, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873)
[M::mem_pestat] mean and std.dev: (323.25, 156.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (121, 147, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042)
[M::mem_pestat] mean and std.dev: (178.91, 94.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1349)
[M::mem_pestat] skip orientation RR
[M::process] read 684348 sequences (100000123 bp) in 2.038 CPU sec, 2.146 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284378, 4, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 190, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778)
[M::mem_pestat] mean and std.dev: (222.78, 86.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 302, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (324.60, 156.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 684714 sequences (100000021 bp) in 1.380 CPU sec, 1.378 real sec...
[M::mem_process_seqs] Processed 683910 reads in 142.030 CPU sec, 4.523 real sec
[M::mem_process_seqs] Processed 683490 reads in 90.432 CPU sec, 2.863 real sec
sorting block 7, #reads = 1070670 mem = 536878960 peak = 845701120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284022, 4, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (106, 106, 257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559)
[M::mem_pestat] mean and std.dev: (146.91, 63.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 710)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 304, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (321.99, 152.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1093)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 221, 258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (42, 402)
[M::mem_pestat] mean and std.dev: (191.75, 44.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 474)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684164 sequences (100000116 bp) in 2.403 CPU sec, 2.534 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284832, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 302, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863)
[M::mem_pestat] mean and std.dev: (320.29, 152.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1084)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683950 sequences (100000143 bp) in 1.340 CPU sec, 1.341 real sec...
[M::mem_process_seqs] Processed 684348 reads in 139.525 CPU sec, 4.449 real sec
sorting block 8, #reads = 1071078 mem = 536878520 peak = 845701120
[M::mem_process_seqs] Processed 684714 reads in 88.999 CPU sec, 2.824 real sec
sorting block 9, #reads = 1071642 mem = 536881990 peak = 899981312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 284509, 0, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (129, 184, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582)
[M::mem_pestat] mean and std.dev: (188.15, 59.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 733)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 305, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874)
[M::mem_pestat] mean and std.dev: (323.89, 154.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1098)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (122, 153, 186)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314)
[M::mem_pestat] mean and std.dev: (134.67, 34.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 378)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683308 sequences (100000282 bp) in 2.268 CPU sec, 2.406 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284251, 0, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (107, 192, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542)
[M::mem_pestat] mean and std.dev: (182.17, 73.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 687)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 306, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881)
[M::mem_pestat] mean and std.dev: (325.60, 155.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1107)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 684164 reads in 139.566 CPU sec, 4.445 real sec
[M::mem_process_seqs] Processed 683950 reads in 89.116 CPU sec, 2.802 real sec
[M::process] read 682948 sequences (100000088 bp) in 2.028 CPU sec, 2.080 real sec...
sorting block 10, #reads = 1072111 mem = 536883315 peak = 899981312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284342, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 307, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (327.42, 156.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683128 sequences (100000082 bp) in 2.149 CPU sec, 2.310 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284636, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 308, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886)
[M::mem_pestat] mean and std.dev: (328.64, 157.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684604 sequences (100000258 bp) in 1.320 CPU sec, 1.317 real sec...
[M::mem_process_seqs] Processed 683308 reads in 139.677 CPU sec, 4.449 real sec
[M::mem_process_seqs] Processed 682948 reads in 90.237 CPU sec, 2.863 real sec
sorting block 11, #reads = 1071625 mem = 536878809 peak = 899981312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284127, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 306, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (327.27, 157.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685624 sequences (100000299 bp) in 2.304 CPU sec, 2.454 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284507, 1, 19)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 302, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866)
[M::mem_pestat] mean and std.dev: (321.16, 153.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1088)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 212, 763)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1945)
[M::mem_pestat] mean and std.dev: (360.26, 276.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2536)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683128 reads in 140.268 CPU sec, 4.456 real sec
[M::process] read 685074 sequences (100000224 bp) in 1.846 CPU sec, 1.862 real sec...
[M::mem_process_seqs] Processed 684604 reads in 87.616 CPU sec, 2.757 real sec
sorting block 12, #reads = 1071307 mem = 536877947 peak = 899981312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285121, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 295, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (314.94, 150.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (115, 169, 220)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430)
[M::mem_pestat] mean and std.dev: (174.27, 60.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 535)
[M::mem_pestat] skip orientation RR
sorting block 13, #reads = 1072999 mem = 536879333 peak = 899981312
[M::process] read 684268 sequences (100000253 bp) in 2.629 CPU sec, 2.793 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284635, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 298, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864)
[M::mem_pestat] mean and std.dev: (318.82, 153.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1086)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685624 reads in 132.663 CPU sec, 4.223 real sec
[M::mem_process_seqs] Processed 685074 reads in 85.554 CPU sec, 2.697 real sec
[M::process] read 683452 sequences (100000217 bp) in 2.197 CPU sec, 2.333 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284079, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 301, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872)
[M::mem_pestat] mean and std.dev: (322.38, 155.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1096)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 225, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1167)
[M::mem_pestat] mean and std.dev: (272.33, 164.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1503)
[M::mem_pestat] skip orientation RR
sorting block 14, #reads = 1072518 mem = 536877364 peak = 899981312
[M::process] read 682440 sequences (100000132 bp) in 2.140 CPU sec, 2.274 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 283948, 0, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 305, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878)
[M::mem_pestat] mean and std.dev: (325.33, 155.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1103)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (125, 167, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (205.69, 91.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 885)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684268 reads in 131.509 CPU sec, 4.201 real sec
[M::mem_process_seqs] Processed 683452 reads in 85.220 CPU sec, 2.692 real sec
sorting block 15, #reads = 1072495 mem = 536879154 peak = 899981312
[M::process] read 682438 sequences (100000126 bp) in 2.415 CPU sec, 2.585 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 283928, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 309, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (329.22, 156.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685656 sequences (100000236 bp) in 2.232 CPU sec, 2.330 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283030, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 308, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (329.19, 156.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 287, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626)
[M::mem_pestat] mean and std.dev: (261.00, 86.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 751)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682440 reads in 134.654 CPU sec, 4.285 real sec
[M::mem_process_seqs] Processed 682438 reads in 86.946 CPU sec, 2.736 real sec
sorting block 16, #reads = 1071448 mem = 536878993 peak = 899981312
[M::process] read 686206 sequences (100000097 bp) in 2.262 CPU sec, 2.428 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284994, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 295, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868)
[M::mem_pestat] mean and std.dev: (317.91, 155.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1092)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285615, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 293, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (314.88, 152.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1078)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685662 sequences (100000234 bp) in 2.105 CPU sec, 2.175 real sec...
[M::mem_process_seqs] Processed 685656 reads in 126.607 CPU sec, 4.043 real sec
sorting block 17, #reads = 1070425 mem = 536877979 peak = 899981312
[M::mem_process_seqs] Processed 686206 reads in 78.672 CPU sec, 2.495 real sec
[M::process] read 684962 sequences (100000101 bp) in 2.366 CPU sec, 2.511 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285634, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 295, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (315.28, 151.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 239, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516)
[M::mem_pestat] mean and std.dev: (222.42, 68.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 632)
[M::mem_pestat] skip orientation RR
sorting block 18, #reads = 1071297 mem = 536877304 peak = 899981312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285022, 0, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (116, 464, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1160)
[M::mem_pestat] mean and std.dev: (263.75, 159.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1508)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 296, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (315.48, 149.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 241, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762)
[M::mem_pestat] mean and std.dev: (280.75, 98.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685662 reads in 125.921 CPU sec, 4.037 real sec
[M::process] read 684476 sequences (100000017 bp) in 2.238 CPU sec, 2.357 real sec...
[M::mem_process_seqs] Processed 684962 reads in 79.741 CPU sec, 2.534 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285142, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 298, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (317.31, 150.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 19, #reads = 1071550 mem = 536877752 peak = 899981312
[M::mem_process_seqs] Processed 684476 reads in 68.642 CPU sec, 2.272 real sec
[M::process] read 684194 sequences (100000185 bp) in 2.866 CPU sec, 3.095 real sec...
sorting block 20, #reads = 1071121 mem = 536877709 peak = 931778560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 284729, 1, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 283, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (254.62, 128.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 295, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (314.62, 148.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 229, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (261.83, 127.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684194 reads in 75.034 CPU sec, 2.431 real sec
[M::process] read 683952 sequences (100000122 bp) in 2.314 CPU sec, 2.485 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284316, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 297, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (317.03, 150.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685280 sequences (100000085 bp) in 1.869 CPU sec, 1.990 real sec...
[M::mem_process_seqs] Processed 683952 reads in 74.124 CPU sec, 2.401 real sec
sorting block 21, #reads = 1070050 mem = 536877838 peak = 931778560
[M::process] read 685918 sequences (100000150 bp) in 1.909 CPU sec, 2.031 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285249, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 296, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (315.62, 150.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (139, 148, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469)
[M::mem_pestat] mean and std.dev: (153.33, 46.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 579)
[M::mem_pestat] skip orientation RR
[M::process] read 685398 sequences (100000272 bp) in 1.270 CPU sec, 1.262 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285618, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 293, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (311.91, 148.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685280 reads in 127.921 CPU sec, 4.019 real sec
[M::process] read 684862 sequences (100000141 bp) in 1.160 CPU sec, 1.164 real sec...
[M::mem_process_seqs] Processed 685918 reads in 78.125 CPU sec, 2.462 real sec
sorting block 22, #reads = 1069428 mem = 536881042 peak = 931778560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285611, 1, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 293, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (311.07, 146.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 286, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (318.00, 114.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RR
[M::process] read 684498 sequences (100000117 bp) in 2.202 CPU sec, 2.370 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284851, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 296, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (314.09, 147.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685398 reads in 124.591 CPU sec, 3.952 real sec
[M::mem_process_seqs] Processed 684862 reads in 78.746 CPU sec, 2.478 real sec
[M::process] read 683966 sequences (100000273 bp) in 1.578 CPU sec, 1.620 real sec...
sorting block 23, #reads = 1071727 mem = 536879356 peak = 931778560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285064, 0, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 149, 178)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (55, 260)
[M::mem_pestat] mean and std.dev: (147.78, 34.00)
[M::mem_pestat] low and high boundaries for proper pairs: (12, 301)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 24, #reads = 1071166 mem = 536880212 peak = 933490688
[M::mem_pestat] (25, 50, 75) percentile: (200, 299, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (317.71, 150.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 683878 sequences (100000034 bp) in 1.983 CPU sec, 2.057 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 284710, 1, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (111, 231, 266)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (182.86, 92.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 731)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 301, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (320.23, 152.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1073)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 685474 sequences (100000275 bp) in 1.290 CPU sec, 1.294 real sec...
[M::mem_process_seqs] Processed 684498 reads in 124.807 CPU sec, 3.958 real sec
[M::mem_process_seqs] Processed 683966 reads in 78.779 CPU sec, 2.485 real sec
sorting block 25, #reads = 1071451 mem = 536880494 peak = 933490688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284947, 1, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (220, 447, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (340.70, 107.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 299, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (319.93, 152.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 294, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590)
[M::mem_pestat] mean and std.dev: (220.88, 68.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685782 sequences (100000152 bp) in 1.796 CPU sec, 1.927 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284884, 0, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 150, 165)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (114, 199)
[M::mem_pestat] mean and std.dev: (145.78, 13.04)
[M::mem_pestat] low and high boundaries for proper pairs: (94, 216)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 295, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859)
[M::mem_pestat] mean and std.dev: (316.98, 153.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1080)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (137, 170, 194)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (23, 308)
[M::mem_pestat] mean and std.dev: (167.85, 33.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 365)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685068 sequences (100000012 bp) in 1.290 CPU sec, 1.292 real sec...
[M::mem_process_seqs] Processed 683878 reads in 128.830 CPU sec, 4.098 real sec
[M::mem_process_seqs] Processed 685474 reads in 82.963 CPU sec, 2.625 real sec
sorting block 26, #reads = 1069621 mem = 536878960 peak = 933490688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285504, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 295, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (314.17, 149.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684458 sequences (100000008 bp) in 2.009 CPU sec, 2.123 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285274, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 295, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (313.27, 147.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684014 sequences (100000144 bp) in 1.280 CPU sec, 1.282 real sec...
[M::mem_process_seqs] Processed 685782 reads in 121.157 CPU sec, 3.838 real sec
[M::mem_process_seqs] Processed 685068 reads in 74.798 CPU sec, 2.365 real sec
sorting block 27, #reads = 1070314 mem = 536877781 peak = 937639936
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285373, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 298, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (316.31, 148.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683572 sequences (100000297 bp) in 1.924 CPU sec, 2.026 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285095, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 301, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (319.28, 150.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1069)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683640 sequences (100000062 bp) in 1.330 CPU sec, 1.333 real sec...
[M::mem_process_seqs] Processed 684458 reads in 125.990 CPU sec, 4.000 real sec
sorting block 28, #reads = 1072215 mem = 536883845 peak = 937639936
[M::mem_process_seqs] Processed 684014 reads in 79.734 CPU sec, 2.514 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 285307, 0, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (226, 243, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (70, 460)
[M::mem_pestat] mean and std.dev: (229.58, 88.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 584)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 303, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (321.17, 150.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1071)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 29, #reads = 1069466 mem = 536879889 peak = 1007271936
[M::process] read 686326 sequences (100000224 bp) in 2.303 CPU sec, 2.437 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284794, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (111, 149, 277)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (193.44, 148.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 786)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 303, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865)
[M::mem_pestat] mean and std.dev: (322.20, 152.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1086)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 683572 reads in 125.881 CPU sec, 3.986 real sec
[M::process] read 686688 sequences (100000281 bp) in 1.534 CPU sec, 1.554 real sec...
[M::mem_process_seqs] Processed 683640 reads in 81.103 CPU sec, 2.551 real sec
sorting block 30, #reads = 1069488 mem = 536879888 peak = 1007271936
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285314, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 293, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869)
[M::mem_pestat] mean and std.dev: (316.46, 155.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1094)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685862 sequences (100000002 bp) in 1.925 CPU sec, 2.051 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285689, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 291, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (313.21, 151.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1073)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (133, 151, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (182.20, 89.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RR
[M::process] read 684828 sequences (100000287 bp) in 1.340 CPU sec, 1.334 real sec...
[M::mem_process_seqs] Processed 686326 reads in 126.241 CPU sec, 4.012 real sec
[M::mem_process_seqs] Processed 686688 reads in 78.434 CPU sec, 2.475 real sec
sorting block 31, #reads = 1069304 mem = 536879010 peak = 1007271936
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285453, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 295, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859)
[M::mem_pestat] mean and std.dev: (316.41, 153.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1080)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683382 sequences (100000142 bp) in 2.179 CPU sec, 2.298 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285552, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 297, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (316.74, 150.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1070)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683006 sequences (100000199 bp) in 1.330 CPU sec, 1.327 real sec...
[M::mem_process_seqs] Processed 685862 reads in 133.040 CPU sec, 4.224 real sec
sorting block 32, #reads = 1071826 mem = 536879254 peak = 1007271936
[M::mem_process_seqs] Processed 684828 reads in 88.111 CPU sec, 2.790 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284762, 0, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 365, 4180)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12224)
[M::mem_pestat] mean and std.dev: (1618.30, 1770.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 16246)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 303, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (320.15, 149.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 33, #reads = 1066635 mem = 536877762 peak = 1007271936
[M::process] read 682604 sequences (100000049 bp) in 1.868 CPU sec, 1.997 real sec...
[M::process] read 47862 sequences (7004167 bp) in 0.340 CPU sec, 0.340 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284463, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 303, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (320.63, 148.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683382 reads in 121.409 CPU sec, 3.898 real sec
[M::mem_process_seqs] Processed 683006 reads in 77.386 CPU sec, 2.472 real sec
sorting block 34, #reads = 1069745 mem = 536879083 peak = 1007271936
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 284209, 0, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 223, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1224)
[M::mem_pestat] mean and std.dev: (359.71, 234.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1579)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 307, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860)
[M::mem_pestat] mean and std.dev: (324.70, 152.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1078)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 19971, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 302, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (322.79, 153.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1075)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682604 reads in 71.948 CPU sec, 2.344 real sec
[M::mem_process_seqs] Processed 47862 reads in 16.051 CPU sec, 0.526 real sec
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 38371786
overall: 69556666368 mem 5364.378 user 186.961 sys 605.994 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:B23KGCJLT4.NA12878_c_0002_gDNA_0004_B23KGCJLT4_1.L006\tPU:L006\tSM:NA12878_c_0002_gDNA_0004_B23KGCJLT4_1\tLB:NA12878_c_0002_gDNA_0004_B23KGCJLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 NA12878_c_0002_gDNA_0004_B23KGCJLT4_1_R1.fastq.gz NA12878_c_0002_gDNA_0004_B23KGCJLT4_1_R2.fastq.gz
[main] Real time: 606.032 sec; CPU: 5551.341 sec
sorting block 35, #reads = 1067933 mem = 535905608 peak = 1007271936
uniq header count 1
merging 36 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 2017789025
output reads: 38556577
bam_mem_sort: 36 calls 18.945 user 0.222 sys 19.228 real
bam_write: 36 calls 9.122 user 0.421 sys 9.531 real
execute: 1 calls 6.870 user 0.426 sys 617.560 real
merge_files: 1 calls 6.854 user 0.404 sys 10.352 real
parse_chunk: 576 calls 24.039 user 6.122 sys 30.540 real
read_chunk: 1557 calls 9.825 user 11.866 sys 135.132 real
sort_block: 1 calls 8.686 user 11.344 sys 132.240 real
write_chunk: 979 calls 6.573 user 0.239 sys 9.720 real
overall: 2369675264 mem 411.409 user 24.225 sys 617.582 real
ls: cannot access '*.cram': No such file or directory