Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ae/ed456dd376bc563d3f7115680e4dd0/NTC_0002_0014_B23KGCJLT4_1/NTC_0002_0014_B23KGCJLT4_1_R1.fastq.gz Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/1e/198a19f94fb5c875a4caa1de819d2e/.command.sh Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ae/ed456dd376bc563d3f7115680e4dd0/NTC_0002_0014_B23KGCJLT4_1/NTC_0002_0014_B23KGCJLT4_1_R2.fastq.gz Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/1e/198a19f94fb5c875a4caa1de819d2e/.command.run ==> STAGING COMPLETE (7 inputs) cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o NTC_0002_0014_B23KGCJLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 2643.282 CPU sec, 95.866 real sec [M::process] read 1336 sequences (201604 bp) in 0.000 CPU sec, 0.002 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 1, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] skip orientation FR as there are not enough pairs [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 1336 reads in 3.016 CPU sec, 0.102 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: bfebfbff 7ffefbff extended: f3bfb7ef fb417ffe amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 1336 overall: 68095635456 mem 2135.960 user 517.582 sys 103.219 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:B23KGCJLT4.NTC_0002_0014_B23KGCJLT4_1.L002\tPU:L002\tSM:NTC_0002_0014_B23KGCJLT4_1\tLB:NTC_0002_0014_B23KGCJLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 NTC_0002_0014_B23KGCJLT4_1_R1.fastq.gz NTC_0002_0014_B23KGCJLT4_1_R2.fastq.gz [main] Real time: 103.254 sec; CPU: 2653.544 sec sorting block 0, #reads = 1354 mem = 754589 peak = 72425472 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: bfebfbff 7ffefbff extended: f3bfb7ef fb417ffe amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 384 algo: util-sort license: sentieon:util=1 output file size: 125277 output reads: 1354 bam_mem_sort: 1 calls 0.000 user 0.000 sys 0.000 real bam_write: 1 calls 0.000 user 0.003 sys 0.003 real execute: 1 calls 0.046 user 0.133 sys 103.379 real merge_files: 1 calls 0.024 user 0.108 sys 0.144 real parse_chunk: 1 calls 0.000 user 0.000 sys 0.001 real read_chunk: 4 calls 0.001 user 0.000 sys 7.246 real sort_block: 1 calls 0.002 user 0.003 sys 7.246 real write_chunk: 1 calls 0.000 user 0.000 sys 0.000 real overall: 259362816 mem 0.068 user 0.183 sys 103.401 real ls: cannot access '*.cram': No such file or directory