.command.log - Workdir - DAQ:SENTIEON_BWAMEM (NA12878_c_0002_gDNA_0003_B23KGCJLT4_1;L001;0001)

File Info

Filename: .command.log
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/d2/e82e12b3584e5174ae226db49e1253/.command.log
Size: 52.1 KB
Task: DAQ:SENTIEON_BWAMEM (NA12878_c_0002_gDNA_0003_B23KGCJLT4_1;L001;0001)
Attempt
Content

  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/d2/e82e12b3584e5174ae226db49e1253/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/66/f4e9d48fe84e8556a0602c6722c296/NA12878_c_0002_gDNA_0003_B23KGCJLT4_1/NA12878_c_0002_gDNA_0003_B23KGCJLT4_1_R2.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/d2/e82e12b3584e5174ae226db49e1253/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/66/f4e9d48fe84e8556a0602c6722c296/NA12878_c_0002_gDNA_0003_B23KGCJLT4_1/NA12878_c_0002_gDNA_0003_B23KGCJLT4_1_R1.fastq.gz
==> STAGING COMPLETE (7 inputs)

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o NA12878_c_0002_gDNA_0003_B23KGCJLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1615.441 CPU sec, 953.967 real sec
[M::process] read 681450 sequences (100000277 bp) in 0.907 CPU sec, 0.912 real sec...
[M::process] read 681830 sequences (100000102 bp) in 1.150 CPU sec, 1.150 real sec...
[M::process] read 681114 sequences (100000301 bp) in 1.150 CPU sec, 1.153 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 283145, 1, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 200, 238)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (82, 342)
[M::mem_pestat] mean and std.dev: (202.36, 54.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 420)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 317, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (335.04, 158.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 216, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511)
[M::mem_pestat] mean and std.dev: (204.67, 68.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 627)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680024 sequences (100000063 bp) in 1.089 CPU sec, 1.083 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282896, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 318, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (340.54, 164.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 283202, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 313, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (333.16, 159.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (137, 279, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605)
[M::mem_pestat] mean and std.dev: (218.50, 73.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 761)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681450 reads in 198.878 CPU sec, 6.361 real sec
[M::mem_process_seqs] Processed 681114 reads in 132.167 CPU sec, 4.248 real sec
sorting block 0, #reads = 1069258 mem = 536878639 peak = 600907776
[M::process] read 680368 sequences (100000088 bp) in 1.455 CPU sec, 1.572 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 281587, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 324, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (343.20, 162.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680024 reads in 68.658 CPU sec, 2.167 real sec
[M::process] read 680792 sequences (100000213 bp) in 1.355 CPU sec, 1.405 real sec...
[M::mem_process_seqs] Processed 681830 reads in 235.595 CPU sec, 7.533 real sec
sorting block 1, #reads = 1068122 mem = 536881180 peak = 729882624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282173, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 319, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (340.11, 162.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680710 sequences (100000017 bp) in 1.493 CPU sec, 1.579 real sec...
[M::process] read 681126 sequences (100000267 bp) in 1.100 CPU sec, 1.104 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282343, 0, 17)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 316, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (339.16, 163.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (347, 451, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (79, 749)
[M::mem_pestat] mean and std.dev: (377.67, 131.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680368 reads in 135.606 CPU sec, 4.278 real sec
[M::mem_process_seqs] Processed 680792 reads in 77.934 CPU sec, 2.468 real sec
sorting block 2, #reads = 1069025 mem = 536880579 peak = 840794112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283283, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 324, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (341.09, 160.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680610 sequences (100000252 bp) in 1.720 CPU sec, 1.815 real sec...
[M::process] read 680040 sequences (100000197 bp) in 1.190 CPU sec, 1.188 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 282588, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 320, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (340.10, 162.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680710 reads in 118.354 CPU sec, 3.758 real sec
sorting block 3, #reads = 1068965 mem = 536878870 peak = 840794112
[M::mem_process_seqs] Processed 681126 reads in 75.589 CPU sec, 2.397 real sec
sorting block 4, #reads = 1068711 mem = 536878718 peak = 895037440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 282582, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 324, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (344.51, 165.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680350 sequences (100000077 bp) in 1.696 CPU sec, 1.794 real sec...
[M::process] read 680432 sequences (100000141 bp) in 1.200 CPU sec, 1.197 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282178, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 324, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (344.04, 164.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680610 reads in 122.412 CPU sec, 3.892 real sec
[M::mem_process_seqs] Processed 680040 reads in 77.435 CPU sec, 2.463 real sec
sorting block 5, #reads = 1068300 mem = 536879130 peak = 895037440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282769, 1, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 245, 258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (21, 416)
[M::mem_pestat] mean and std.dev: (205.44, 77.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 514)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 320, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (342.36, 165.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (124, 184, 1303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3661)
[M::mem_pestat] mean and std.dev: (961.75, 1174.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5659)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680854 sequences (100000054 bp) in 1.875 CPU sec, 2.010 real sec...
[M::process] read 681152 sequences (100000008 bp) in 1.220 CPU sec, 1.227 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282114, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 321, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (342.39, 165.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680350 reads in 125.042 CPU sec, 3.976 real sec
[M::mem_process_seqs] Processed 680432 reads in 78.992 CPU sec, 2.506 real sec
sorting block 6, #reads = 1068173 mem = 536878987 peak = 895037440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282982, 4, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 323, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (342.11, 163.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (140, 211, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590)
[M::mem_pestat] mean and std.dev: (196.31, 84.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 740)
[M::mem_pestat] skip orientation RR
[M::process] read 680020 sequences (100000000 bp) in 1.968 CPU sec, 2.077 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 283597, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 175, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624)
[M::mem_pestat] mean and std.dev: (211.23, 100.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 321, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (340.41, 162.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 680036 sequences (100000247 bp) in 1.260 CPU sec, 1.257 real sec...
[M::mem_process_seqs] Processed 680854 reads in 121.428 CPU sec, 3.860 real sec
[M::mem_process_seqs] Processed 681152 reads in 76.042 CPU sec, 2.411 real sec
sorting block 7, #reads = 1067893 mem = 536881582 peak = 895037440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282780, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 326, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (346.34, 166.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680546 sequences (100000091 bp) in 1.934 CPU sec, 2.071 real sec...
[M::process] read 680456 sequences (100000211 bp) in 1.280 CPU sec, 1.280 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282308, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 324, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (345.02, 165.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680020 reads in 124.828 CPU sec, 3.973 real sec
sorting block 8, #reads = 1069391 mem = 536884608 peak = 895037440
[M::mem_process_seqs] Processed 680036 reads in 78.179 CPU sec, 2.489 real sec
sorting block 9, #reads = 1067900 mem = 536880183 peak = 942936064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 283146, 0, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 265, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518)
[M::mem_pestat] mean and std.dev: (224.22, 58.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 637)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 319, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (342.60, 166.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 248, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (253.53, 102.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681088 sequences (100000158 bp) in 2.324 CPU sec, 2.452 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282846, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 322, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (343.78, 166.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 320, 630)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1388)
[M::mem_pestat] mean and std.dev: (369.45, 270.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1767)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680546 reads in 123.733 CPU sec, 3.926 real sec
[M::process] read 681324 sequences (100000172 bp) in 1.318 CPU sec, 1.312 real sec...
[M::mem_process_seqs] Processed 680456 reads in 78.382 CPU sec, 2.473 real sec
sorting block 10, #reads = 1069224 mem = 536880051 peak = 942936064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 282594, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 320, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (341.61, 165.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680678 sequences (100000131 bp) in 2.046 CPU sec, 2.176 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282962, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 315, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (336.43, 161.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680450 sequences (100000197 bp) in 1.310 CPU sec, 1.306 real sec...
[M::mem_process_seqs] Processed 681088 reads in 121.468 CPU sec, 3.861 real sec
sorting block 11, #reads = 1069156 mem = 536878344 peak = 942936064
[M::process] read 680442 sequences (100000125 bp) in 1.464 CPU sec, 1.471 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 282542, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 317, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (340.12, 164.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 190, 1063)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2869)
[M::mem_pestat] mean and std.dev: (545.90, 645.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3772)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680678 reads in 71.800 CPU sec, 2.467 real sec
[M::mem_process_seqs] Processed 681324 reads in 165.457 CPU sec, 5.447 real sec
sorting block 12, #reads = 1068939 mem = 536878420 peak = 942936064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 282486, 1, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (114, 181, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (222.11, 142.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 317, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (339.95, 163.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 681050 sequences (100000039 bp) in 1.902 CPU sec, 2.014 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 282623, 3, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 188, 832)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2144)
[M::mem_pestat] mean and std.dev: (379.15, 295.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2800)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 317, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (340.21, 164.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (123, 198, 246)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492)
[M::mem_pestat] mean and std.dev: (167.92, 64.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 615)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681868 sequences (100000056 bp) in 1.250 CPU sec, 1.255 real sec...
[M::mem_process_seqs] Processed 680450 reads in 137.543 CPU sec, 4.378 real sec
[M::mem_process_seqs] Processed 680442 reads in 79.917 CPU sec, 2.526 real sec
sorting block 13, #reads = 1068505 mem = 536878315 peak = 942936064
[M::process] read 682082 sequences (100000137 bp) in 2.003 CPU sec, 2.149 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 283501, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 312, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (331.28, 158.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682010 sequences (100000127 bp) in 1.306 CPU sec, 1.303 real sec...
[M::mem_process_seqs] Processed 681868 reads in 67.669 CPU sec, 2.131 real sec
sorting block 14, #reads = 1069316 mem = 536878850 peak = 942936064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282990, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 315, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (337.80, 163.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (122, 207, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (239.33, 158.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 910)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283359, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 309, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (328.65, 156.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1116)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681050 reads in 193.293 CPU sec, 6.141 real sec
[M::process] read 681276 sequences (100000068 bp) in 1.742 CPU sec, 1.749 real sec...
[M::mem_process_seqs] Processed 682082 reads in 90.990 CPU sec, 2.889 real sec
sorting block 15, #reads = 1068645 mem = 536880988 peak = 942936064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 283467, 2, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (109, 162, 225)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457)
[M::mem_pestat] mean and std.dev: (173.50, 54.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 573)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 308, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886)
[M::mem_pestat] mean and std.dev: (328.47, 156.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 267, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (260.44, 143.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680954 sequences (100000021 bp) in 2.190 CPU sec, 2.343 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282942, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 307, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869)
[M::mem_pestat] mean and std.dev: (326.84, 153.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1090)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682010 reads in 126.478 CPU sec, 4.023 real sec
[M::process] read 681274 sequences (100000197 bp) in 1.458 CPU sec, 1.461 real sec...
[M::mem_process_seqs] Processed 681276 reads in 78.009 CPU sec, 2.469 real sec
sorting block 16, #reads = 1069960 mem = 536883130 peak = 942936064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 283155, 0, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (106, 119, 164)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280)
[M::mem_pestat] mean and std.dev: (139.50, 43.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 338)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 310, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874)
[M::mem_pestat] mean and std.dev: (329.20, 153.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1096)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (133, 198, 271)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547)
[M::mem_pestat] mean and std.dev: (213.06, 113.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 685)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681620 sequences (100000257 bp) in 2.042 CPU sec, 2.197 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283040, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 311, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (332.50, 159.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680954 reads in 124.674 CPU sec, 3.968 real sec
sorting block 17, #reads = 1069476 mem = 536883509 peak = 942936064
[M::process] read 682144 sequences (100000015 bp) in 1.569 CPU sec, 1.586 real sec...
[M::mem_process_seqs] Processed 681274 reads in 79.275 CPU sec, 2.499 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 283555, 0, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (110, 114, 168)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284)
[M::mem_pestat] mean and std.dev: (121.44, 26.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 342)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 310, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878)
[M::mem_pestat] mean and std.dev: (328.60, 154.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 18, #reads = 1067718 mem = 536878747 peak = 968273920
[M::process] read 681738 sequences (100000010 bp) in 2.231 CPU sec, 2.385 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 283634, 0, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 178, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556)
[M::mem_pestat] mean and std.dev: (217.31, 72.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 690)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 304, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (323.18, 152.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 681620 reads in 123.040 CPU sec, 3.924 real sec
[M::mem_process_seqs] Processed 682144 reads in 78.108 CPU sec, 2.467 real sec
[M::process] read 680912 sequences (100000001 bp) in 1.934 CPU sec, 2.045 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 283888, 3, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 307, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874)
[M::mem_pestat] mean and std.dev: (327.19, 155.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 234, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (234.40, 88.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 755)
[M::mem_pestat] skip orientation RR
sorting block 19, #reads = 1069034 mem = 536880356 peak = 968273920
[M::process] read 680750 sequences (100000027 bp) in 2.389 CPU sec, 2.550 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282737, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 312, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881)
[M::mem_pestat] mean and std.dev: (331.16, 155.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1105)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681738 reads in 125.506 CPU sec, 4.025 real sec
[M::mem_process_seqs] Processed 680912 reads in 80.215 CPU sec, 2.545 real sec
sorting block 20, #reads = 1070134 mem = 536877066 peak = 968273920
[M::process] read 681168 sequences (100000003 bp) in 1.950 CPU sec, 2.070 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282917, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 316, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (335.87, 159.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681468 sequences (100000178 bp) in 1.695 CPU sec, 1.800 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 282965, 1, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 261, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453)
[M::mem_pestat] mean and std.dev: (199.12, 56.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 549)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 313, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (336.16, 162.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 417, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1174)
[M::mem_pestat] mean and std.dev: (314.15, 143.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1511)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680750 reads in 124.954 CPU sec, 3.980 real sec
[M::mem_process_seqs] Processed 681168 reads in 81.687 CPU sec, 2.573 real sec
[M::process] read 681754 sequences (100000178 bp) in 1.453 CPU sec, 1.503 real sec...
sorting block 21, #reads = 1069048 mem = 536878870 peak = 968273920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283055, 0, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 314, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (332.98, 158.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 224, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (199.07, 85.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 699)
[M::mem_pestat] skip orientation RR
sorting block 22, #reads = 1067413 mem = 536878778 peak = 968273920
[M::process] read 681262 sequences (100000033 bp) in 2.002 CPU sec, 2.151 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 283207, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 307, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (326.98, 155.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1100)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681468 reads in 120.204 CPU sec, 3.833 real sec
[M::process] read 680762 sequences (100000099 bp) in 1.313 CPU sec, 1.320 real sec...
[M::mem_process_seqs] Processed 681754 reads in 76.741 CPU sec, 2.432 real sec
sorting block 23, #reads = 1069679 mem = 536878576 peak = 968273920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 283293, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 310, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (329.24, 156.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1099)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680512 sequences (100000025 bp) in 2.012 CPU sec, 2.133 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 282717, 1, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 164, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 372)
[M::mem_pestat] mean and std.dev: (169.69, 49.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 446)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 311, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875)
[M::mem_pestat] mean and std.dev: (330.25, 154.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 331, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (62, 567)
[M::mem_pestat] mean and std.dev: (299.18, 56.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 668)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681262 reads in 121.038 CPU sec, 3.851 real sec
[M::process] read 681188 sequences (100000202 bp) in 1.350 CPU sec, 1.345 real sec...
[M::mem_process_seqs] Processed 680762 reads in 76.857 CPU sec, 2.429 real sec
sorting block 24, #reads = 1069763 mem = 536879817 peak = 968273920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282549, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 315, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (335.68, 159.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682356 sequences (100000249 bp) in 2.039 CPU sec, 2.160 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 283068, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 314, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (337.20, 163.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682118 sequences (100000013 bp) in 1.320 CPU sec, 1.325 real sec...
[M::mem_process_seqs] Processed 680512 reads in 128.828 CPU sec, 4.096 real sec
[M::mem_process_seqs] Processed 681188 reads in 82.049 CPU sec, 2.600 real sec
sorting block 25, #reads = 1067280 mem = 536881059 peak = 968273920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283577, 1, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 311, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (335.94, 165.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 194, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (205.33, 71.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RR
[M::process] read 681148 sequences (100000018 bp) in 2.067 CPU sec, 2.202 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 283496, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 310, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (332.17, 160.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 222, 275)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (86, 401)
[M::mem_pestat] mean and std.dev: (218.11, 47.17)
[M::mem_pestat] low and high boundaries for proper pairs: (23, 464)
[M::mem_pestat] skip orientation RR
[M::process] read 680800 sequences (100000071 bp) in 1.320 CPU sec, 1.321 real sec...
[M::mem_process_seqs] Processed 682356 reads in 122.692 CPU sec, 3.898 real sec
sorting block 26, #reads = 1066896 mem = 536879471 peak = 968273920
[M::mem_process_seqs] Processed 682118 reads in 76.757 CPU sec, 2.430 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 283008, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 314, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (336.21, 161.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 382, 560)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1186)
[M::mem_pestat] mean and std.dev: (384.00, 166.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1499)
[M::mem_pestat] skip orientation RR
sorting block 27, #reads = 1070275 mem = 536880270 peak = 968273920
[M::process] read 680152 sequences (100000051 bp) in 2.185 CPU sec, 2.347 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282893, 0, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 314, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (335.10, 159.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (295, 777, 1338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3424)
[M::mem_pestat] mean and std.dev: (769.43, 510.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4467)
[M::mem_pestat] skip orientation RR
[M::process] read 504458 sequences (74173092 bp) in 1.080 CPU sec, 1.085 real sec...
[M::mem_process_seqs] Processed 681148 reads in 124.459 CPU sec, 3.962 real sec
[M::mem_process_seqs] Processed 680800 reads in 78.957 CPU sec, 2.508 real sec
sorting block 28, #reads = 1067552 mem = 536877089 peak = 968273920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282572, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 315, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (336.63, 159.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (282, 772, 901)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2139)
[M::mem_pestat] mean and std.dev: (564.83, 301.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2758)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 209763, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 316, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (338.92, 163.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680152 reads in 108.582 CPU sec, 3.528 real sec
[M::mem_process_seqs] Processed 504458 reads in 60.114 CPU sec, 1.950 real sec
sorting block 29, #reads = 1068674 mem = 536879252 peak = 968273920
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 32512716
overall: 69708685312 mem 4621.522 user 241.645 sys 1066.202 real
sorting block 30, #reads = 608751 mem = 306168005 peak = 1008099328
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:B23KGCJLT4.NA12878_c_0002_gDNA_0003_B23KGCJLT4_1.L001\tPU:L001\tSM:NA12878_c_0002_gDNA_0003_B23KGCJLT4_1\tLB:NA12878_c_0002_gDNA_0003_B23KGCJLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 NA12878_c_0002_gDNA_0003_B23KGCJLT4_1_R1.fastq.gz NA12878_c_0002_gDNA_0003_B23KGCJLT4_1_R2.fastq.gz
[main] Real time: 1066.250 sec; CPU: 4863.180 sec
uniq header count 1
merging 31 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 1636482020
output reads: 32671176
bam_mem_sort: 31 calls 13.633 user 0.226 sys 13.932 real
bam_write: 31 calls 9.927 user 0.378 sys 10.396 real
execute: 1 calls 6.044 user 0.408 sys 1075.036 real
merge_files: 1 calls 6.025 user 0.389 sys 8.201 real
parse_chunk: 490 calls 18.310 user 4.662 sys 23.360 real
read_chunk: 1323 calls 9.447 user 10.547 sys 108.655 real
sort_block: 1 calls 7.739 user 9.991 sys 105.578 real
write_chunk: 831 calls 5.717 user 0.217 sys 7.537 real
overall: 2410553344 mem 320.068 user 21.316 sys 1075.058 real
ls: cannot access '*.cram': No such file or directory